freshwater_medium_for_soil_aoa

CultureMech:002190 bacterial DEFINED LIQUID
Original name: FRESHWATER MEDIUM FOR SOIL AOA

Overview

External ontology
mediadive.medium:J1004 (source link unavailable) — FRESHWATER MEDIUM FOR SOIL AOA
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1004

Composition

flowchart LR
    CultureMech_002190["freshwater_medium_for_soil_aoa"]
    ing0_NaCl["NaCl"]
    CultureMech_002190 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_002190 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002190 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002190 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002190 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_002190 --> ing5_FeNa_EDTA
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002190 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_NH4Cl["NH4Cl"]
    CultureMech_002190 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002190 --> ing8_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing8_Sodium_pyruvate -.-> CHEBI_50144
    ing9_HCl["HCl"]
    CultureMech_002190 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_H3BO3["H3BO3"]
    CultureMech_002190 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002190 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002190 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002190 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002190 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002190 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002190 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Biotin["Biotin"]
    CultureMech_002190 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_002190 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002190 --> ing19_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing20_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002190 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Riboflavin["Riboflavin"]
    CultureMech_002190 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002190 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002190 --> ing23_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_002190 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002190 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26_Choline_chloride["Choline chloride"]
    CultureMech_002190 --> ing26_Choline_chloride
    CHEBI_133341["CHEBI:133341"]
    ing26_Choline_chloride -.-> CHEBI_133341
    ing27_NaOH["NaOH"]
    CultureMech_002190 --> ing27_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing27_NaOH -.-> CHEBI_32145
    ing28_HEPES["HEPES"]
    CultureMech_002190 --> ing28_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing28_HEPES -.-> CHEBI_46756

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.983284 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.491642 G_PER_L CHEBI:32588
potassium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.393314 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.196657 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0983284 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 FeNa-EDTA 1 G_PER_L
7 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
9 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1 G_PER_L CHEBI:50144
sodium pyruvate
10 HCl
HCl 36.461 g/mol
12.5 G_PER_L CHEBI:17883
hydrogen chloride
11 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
19 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
20 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
21 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
22 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
23 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
24 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
25 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
26 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
27 Choline chloride
C5H14NO.Cl 139.626 g/mol
2 G_PER_L CHEBI:133341
choline chloride
28 NaOH
HO.Na 39.997 g/mol
12 G_PER_L CHEBI:32145
sodium hydroxide
29 HEPES 119.2 G_PER_L CHEBI:46756
HEPES

Preparation

  1. Autoclave Basic FWM solution and aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order:
  2. Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking.
  3. Adjust pH to 7.0 with KOH.
  4. Dissolve NaOH in 300 ml distilled water. While stirring vigorously, slowly add HEPES until completely dissolved. Fill up to 450 ml with distilled water. If 1 ml of this solution is added to 100ml of the medium (containing all additive but HEPES), pH should be around 7.6 at 30C. Otherwise, adjust pH of the HEPES solution with 10 N NaOH or conc. HCl. Finally fill up to 500 ml with distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.287456Z mediadive-import Imported from MediaDive Source: JCM, ID: J1004
2026-03-13T06:19:11.110068Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002190
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.860194+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FRESHWATER MEDIUM FOR SOIL AOA" to "freshwater_medium_for_soil_aoa" for consistent matching
2026-06-05T07:41:05.492253+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.808838+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.