Overview
- External ontology
- mediadive.medium:J100 (source link unavailable) — ALKALINE NUTRIENT AGAR
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- JCM Medium 100 / Alkaline Nutrient Agar uses the same Nutrient Agar basal formulation as JCM Medium 74, then after autoclaving adjusts the final medium to pH 10.0 with sterile 10% Na2CO3 solution. TOGO M92 and MediaDive J100 report the same JCM recipe.
Composition
flowchart LR
CultureMech_002194["alkaline_nutrient_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_002194 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Peptone["Peptone"]
CultureMech_002194 --> ing1_Peptone
ing2_Beef_extract["Beef extract"]
CultureMech_002194 --> ing2_Beef_extract
ing3_Agar["Agar"]
CultureMech_002194 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
MediaDive J100 / JCM Medium 100 lists 1.0 L Distilled water. |
| 2 | Peptone | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
MediaDive J100 / JCM Medium 100 lists 5.0 g/L Peptone. |
| 3 | Beef extract | 3.0 G_PER_L |
FOODON:03302088
Beef extract
|
MediaDive J100 / JCM Medium 100 lists 3.0 g/L Beef extract. |
| 4 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
MediaDive J100 / JCM Medium 100 lists 15.0 g/L Agar. |
Preparation
- Dissolve 5.0 g/L peptone, 3.0 g/L beef extract, and 15.0 g/L agar in 1.0 L distilled water.
- Autoclave the basal nutrient agar.
- After autoclaving, adjust pH to 10.0 with sterile 10% Na2CO3 solution.
Solutions
10% Na2CO3 solution
Dose: variable VARIABLE
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 10.0 PERCENT_W_V | MediaDive J100 / JCM Medium 100 specifies the pH-adjusting sodium carbonate solution as 10.0% w/v. |
MediaDive J100 / JCM Medium 100 uses sterile 10% Na2CO3 solution to adjust the final medium to pH 10.0; the source does not specify a fixed addition volume.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.730002Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J100 |
| 2026-03-13T06:19:11.134949Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002194 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.888439+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE NUTRIENT AGAR" to "alkaline_nutrient_agar" for consistent matching |
| 2026-06-05T07:40:16.409485+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m92_alkaline_nutrient_score15.py | RESOLVED_MEDIADIVE_J100_ALKALINE_NUTRIENT_AGAR | Added source 1.0 L distilled water, grounded peptone and beef extract, structured sterile 10% Na2CO3 as the variable pH adjuster, and linked the JCM Medium 74 parent plus TOGO M92 source duplicate. |