nasu_iron_reducer_medium

CultureMech:002199 bacterial COMPLEX LIQUID
Original name: NASU IRON REDUCER MEDIUM

Overview

External ontology
mediadive.medium:J1014 (source link unavailable) — NASU IRON REDUCER MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1014

Composition

flowchart LR
    CultureMech_002199["nasu_iron_reducer_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002199 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002199 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002199 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002199 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002199 --> ing4_Yeast_extract
    ing5_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_002199 --> ing5_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing5_L_Cysteine_HCl -.-> CHEBI_91247
    ing6_FeCl2["FeCl2"]
    CultureMech_002199 --> ing6_FeCl2
    CHEBI_30812["CHEBI:30812"]
    ing6_FeCl2 -.-> CHEBI_30812
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002199 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002199 --> ing8_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing8_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002199 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002199 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002199 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_002199 --> ing12_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing12_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002199 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_Biotin["Biotin"]
    CultureMech_002199 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_Folic_acid["Folic acid"]
    CultureMech_002199 --> ing15_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing15_Folic_acid -.-> CHEBI_27470
    ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002199 --> ing16_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing17_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002199 --> ing17_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing17_Thiamine_HCl -.-> CHEBI_49105
    ing18_Riboflavin["Riboflavin"]
    CultureMech_002199 --> ing18_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing18_Riboflavin -.-> CHEBI_17015
    ing19_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002199 --> ing19_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing19_Nicotinic_acid -.-> CHEBI_15940
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002199 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_002199 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843
    ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002199 --> ing22_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing23_Lipoic_acid["Lipoic acid"]
    CultureMech_002199 --> ing23_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing23_Lipoic_acid -.-> CHEBI_16494

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.193986 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
2.9098 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.484966 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.242483 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Yeast extract 0.484966 G_PER_L
6 L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
10 G_PER_L CHEBI:91247
L-cysteine hydrochloride
7 FeCl2
Cl2Fe 126.751 g/mol
10 G_PER_L CHEBI:30812
iron dichloride
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.8 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
4.5 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.05 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
0.03 G_PER_L CHEBI:87020
vanadyl sulfate hydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.01 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
16 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
17 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
18 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
19 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
20 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
21 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
22 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
23 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
24 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly and adjust pH to 6.0. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere):
  2. Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.323700Z mediadive-import Imported from MediaDive Source: JCM, ID: J1014
2026-03-13T06:19:11.184373Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002199
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.944970+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NASU IRON REDUCER MEDIUM" to "nasu_iron_reducer_medium" for consistent matching
2026-06-05T07:41:39.169668+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.367135+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.