mmjhs_2_medium

CultureMech:002202 bacterial DEFINED LIQUID
Original name: MMJHS-2 MEDIUM

Overview

External ontology
mediadive.medium:J1019 (source link unavailable) — MMJHS-2 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1019

Composition

flowchart LR
    CultureMech_002202["mmjhs_2_medium"]
    ing0_NaNO3["NaNO3"]
    CultureMech_002202 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002202 --> ing1_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing1_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing2_NaHCO3["NaHCO3"]
    CultureMech_002202 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Sulfur["Sulfur"]
    CultureMech_002202 --> ing3_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing3_Sulfur -.-> CHEBI_26833
    ing4_NaCl["NaCl"]
    CultureMech_002202 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002202 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002202 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_KCl["KCl"]
    CultureMech_002202 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NH4Cl["NH4Cl"]
    CultureMech_002202 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_K2HPO4["K2HPO4"]
    CultureMech_002202 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002202 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002202 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002202 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002202 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002202 --> ing14_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002202 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002202 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002202 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002202 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002202 --> ing19_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing19_AlK_SO4_2 -.-> CHEBI_86463
    ing20_H3BO3["H3BO3"]
    CultureMech_002202 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002202 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
2 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
3 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
4 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom
5 NaCl
Cl.Na 58.443 g/mol
25.7525 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 24.7525, 1.0]
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.15842 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.36634 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.36634, 3.0]
8 KCl
Cl.K 74.551 g/mol
0.49505 G_PER_L CHEBI:32588
potassium chloride
9 NH4Cl
Cl.H4N 53.492 g/mol
0.247525 G_PER_L CHEBI:31206
ammonium chloride
10 K2HPO4
HO4P.2K 174.174 g/mol
0.138614 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.793069 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.693069, 0.1]
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025990099000000003 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.000990099, 0.025]
13 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0012900989999999998 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 [Merged 2 duplicates: 0.000990099, 0.0003]
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components, except sulfur and NaHCO3, and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize 8% (w/v) NaHCO3 solution and add to the medium. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of the medium with 0.2 g sulfur in 100 ml serum bottle) and seal with butyl rubber stoppers. Replace gas phase with a H2-CO2 (4:1, v/v) gas mixture and check medium pH to be 6.0. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.336626Z mediadive-import Imported from MediaDive Source: JCM, ID: J1019
2026-03-13T03:09:00.828392Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:11.211157Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002202
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.978244+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJHS-2 MEDIUM" to "mmjhs_2_medium" for consistent matching
2026-06-05T07:41:35.590191+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.320172+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.