Overview
- External ontology
- mediadive.medium:J101 (source link unavailable) — BACILLUS ACIDOCALDARIUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=101
Composition
flowchart LR
CultureMech_002203["bacillus_acidocaldarius_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002203 --> ing0_Yeast_extract
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002203 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002203 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002203 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_002203 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Glucose["Glucose"]
CultureMech_002203 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar["Agar"]
CultureMech_002203 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 | Agar | 40 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 3.0-4.0.
- Autoclave Solutions A and B separately, and mix aseptically.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.731313Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J101 |
| 2026-03-13T06:19:11.217744Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002203 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.985990+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BACILLUS ACIDOCALDARIUS MEDIUM" to "bacillus_acidocaldarius_medium" for consistent matching |
| 2026-06-05T07:40:16.433614+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.703086+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |