nutrient_agar_with_25_soil_extract

CultureMech:002213 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIENT AGAR WITH 25% SOIL EXTRACT

Overview

External ontology
mediadive.medium:J102 (source link unavailable) — NUTRIENT AGAR WITH 25% SOIL EXTRACT
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=102

Composition

flowchart LR
    CultureMech_002213["nutrient_agar_with_25_soil_extract"]
    ing0_Peptone["Peptone"]
    CultureMech_002213 --> ing0_Peptone
    ing1_Beef_extract["Beef extract"]
    CultureMech_002213 --> ing1_Beef_extract
    ing2_Agar["Agar"]
    CultureMech_002213 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Tap_water["Tap water"]
    CultureMech_002213 --> ing3_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Tap_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Beef extract 3 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
4 Tap water
H2O 18.015 g/mol
750 G_PER_L CHEBI:15377
water

Preparation

  1. Adjust pH to 7.0.
  2. Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.733110Z mediadive-import Imported from MediaDive Source: JCM, ID: J102
2026-03-13T06:19:11.292628Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002213
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.069395+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIENT AGAR WITH 25% SOIL EXTRACT" to "nutrient_agar_with_25_soil_extract" for consistent matching
2026-06-05T07:41:40.148776+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.