Overview
- External ontology
- mediadive.medium:J1030 (source link unavailable) — DISSULFURIMICROBIUM HYDROTHERMALE MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1030
Composition
flowchart LR
CultureMech_002214["dissulfurimicrobium_hydrothermale_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002214 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KCl["KCl"]
CultureMech_002214 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002214 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002214 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_002214 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Sulfur["Sulfur"]
CultureMech_002214 --> ing5_Sulfur
CHEBI_26833["CHEBI:26833"]
ing5_Sulfur -.-> CHEBI_26833
ing6_NaHCO3["NaHCO3"]
CultureMech_002214 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002214 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002214 --> ing8_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002214 --> ing9_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002214 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002214 --> ing11_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing11_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002214 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002214 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_Na2SeO4["Na2SeO4"]
CultureMech_002214 --> ing14_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing14_Na2SeO4 -.-> CHEBI_77775
ing15_H3BO3["H3BO3"]
CultureMech_002214 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002214 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_SrSO4["SrSO4"]
CultureMech_002214 --> ing17_SrSO4
ing18_CrK_SO4_2_x_12_H2O["CrK(SO4)2 x 12 H2O"]
CultureMech_002214 --> ing18_CrK_SO4_2_x_12_H2O
ing19_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
CultureMech_002214 --> ing19_AlCl3_x_6_H2O
CHEBI_30115["CHEBI:30115"]
ing19_AlCl3_x_6_H2O -.-> CHEBI_30115
ing20_BaCl2["BaCl2"]
CultureMech_002214 --> ing20_BaCl2
CHEBI_63317["CHEBI:63317"]
ing20_BaCl2 -.-> CHEBI_63317
ing21_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
CultureMech_002214 --> ing21_Na2SiO3_x_9_H2O
CHEBI_132108["CHEBI:132108"]
ing21_Na2SiO3_x_9_H2O -.-> CHEBI_132108
ing22_KBr["KBr"]
CultureMech_002214 --> ing22_KBr
CHEBI_32030["CHEBI:32030"]
ing22_KBr -.-> CHEBI_32030
ing23_KI["KI"]
CultureMech_002214 --> ing23_KI
CHEBI_8346["CHEBI:8346"]
ing23_KI -.-> CHEBI_8346
ing24_Na2SO4["Na2SO4"]
CultureMech_002214 --> ing24_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing24_Na2SO4 -.-> CHEBI_32149
ing25_Biotin["Biotin"]
CultureMech_002214 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_002214 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002214 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_002214 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_002214 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_002214 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.402439 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.402439 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.402439 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.402439 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.402439 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Sulfur
S 32.067 g/mol
|
6.09756 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.43902 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.0784 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 9 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0238 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 10 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0166 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.00242 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 12 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.00549 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.00945 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.00062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0198 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 | SrSO4 | 0.00092 G_PER_L | ||
| 19 | CrK(SO4)2 x 12 H2O | 0.0005 G_PER_L | ||
| 20 |
AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
|
0.00241 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
|
| 21 |
BaCl2
Ba.2Cl 208.234 g/mol
|
0.00208 G_PER_L |
CHEBI:63317
barium chloride
|
|
| 22 |
Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
|
0.0142 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
|
| 23 |
KBr
Br.K 119.002 g/mol
|
0.0119 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 24 |
KI
I.K 166.002 g/mol
|
0.0166 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 25 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.071 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components, except sulfur, bring to a boil and cool down under a CO2 (100%) gas stream. While gassing, add the following components to the medium:
- Bubble CO2 for 3 - 4 min and check pH to be 6.5 - 6.8. Readjust pH to the range, if necessary. Then, suspend ferrihydrite (see below) in the medium and dispense the suspension into culture vessels (e.g., 10 ml in Hungate tubes) under a CO2 gas stream. Add appropriate amount of sulfur (powder). Seal the culture vessels with butyl rubber stoppers and autoclave at 105C for 1 hr.
- <I>Ferrihydrite:</I> Slowly titrate 320 ml of FeCl3 x 6H2O solution (60.0 g/L) with 10% (w/v) NaOH to pH 7.0-7.5 with agitation on a magnetic stirrer (approximately 80-100 ml NaOH solution). Remove ferrihydrite into a container, cover with water and store at room temperature overnight. Remove supernatant after centrifugation at 2000 rpm for 5 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.373903Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1030 |
| 2026-01-28T19:48:32.331618Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:11.304115Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002214 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.081956+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DISSULFURIMICROBIUM HYDROTHERMALE MEDIUM" to "dissulfurimicrobium_hydrothermale_medium" for consistent matching |
| 2026-06-05T07:40:58.760194+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.736223+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:56.415189+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |