lb_agar_with_mnso_sub_4_sub

CultureMech:002225 bacterial COMPLEX SOLID_AGAR
Original name: LB AGAR WITH MnSO<sub>4</sub>

Overview

External ontology
mediadive.medium:J1040 (source link unavailable) — LB AGAR WITH MnSO<sub>4</sub>
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1040

Composition

flowchart LR
    CultureMech_002225["lb_agar_with_mnso_sub_4_sub"]
    ing0_Tryptone["Tryptone"]
    CultureMech_002225 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002225 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_002225 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002225 --> ing3_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing3_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing4_Agar["Agar"]
    CultureMech_002225 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Tryptone 10 G_PER_L
2 Yeast extract 5 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
4 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.01 G_PER_L CHEBI:86360
manganese(II) sulfate
5 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.415614Z mediadive-import Imported from MediaDive Source: JCM, ID: J1040
2026-03-13T06:19:11.418154Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002225
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.208493+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB AGAR WITH MnSO<sub>4</sub>" to "lb_agar_with_mnso_sub_4_sub" for consistent matching
2026-06-05T07:41:10.817048+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.491177+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.