ml_15_medium

CultureMech:002228 bacterial COMPLEX LIQUID
Original name: ML-15 MEDIUM

Overview

External ontology
mediadive.medium:J1044 (source link unavailable) — ML-15 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1044

Composition

flowchart LR
    CultureMech_002228["ml_15_medium"]
    ing0_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002228 --> ing0_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing0_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing1_NaHCO3["NaHCO3"]
    CultureMech_002228 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_Na2CO3["Na2CO3"]
    CultureMech_002228 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_NaCl["NaCl"]
    CultureMech_002228 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002228 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002228 --> ing5_Yeast_extract

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
3.72549 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
2 NaHCO3
CHO3.Na 84.006 g/mol
6.66667 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 Na2CO3
CO3.2Na 105.988 g/mol
31.3725 G_PER_L CHEBI:29377
sodium carbonate
4 NaCl
Cl.Na 58.443 g/mol
68.6274 G_PER_L CHEBI:26710
sodium chloride
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.980392 G_PER_L CHEBI:62946
ammonium sulfate
6 Yeast extract 20 G_PER_L

Preparation

  1. Mix thoroughly and autoclave. After cooling, add the following solution (autoclaved).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.431179Z mediadive-import Imported from MediaDive Source: JCM, ID: J1044
2026-03-13T06:19:11.447425Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002228
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.239559+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ML-15 MEDIUM" to "ml_15_medium" for consistent matching
2026-06-05T07:41:35.173794+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.211362+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.