Overview
- External ontology
- mediadive.medium:J1046 (source link unavailable) — JCM MEDIUM No. 1046
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1046
Composition
flowchart LR
CultureMech_002230["jcm_medium_no_1046"]
ing0_NaCl["NaCl"]
CultureMech_002230 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002230 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002230 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NaHCO3["NaHCO3"]
CultureMech_002230 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_NH4Cl["NH4Cl"]
CultureMech_002230 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_002230 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002230 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002230 --> ing7_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing7_Nitrilotriacetic_acid -.-> CHEBI_44557
ing8_Yeast_extract["Yeast extract"]
CultureMech_002230 --> ing8_Yeast_extract
ing9_Glucose["Glucose"]
CultureMech_002230 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_Agar["Agar"]
CultureMech_002230 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002230 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002230 --> ing12_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing12_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing13_H3BO3["H3BO3"]
CultureMech_002230 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002230 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_Thiamine_HCl["Thiamine HCl"]
CultureMech_002230 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
79.9201 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.11988 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0609391 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.1958 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.849151 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.47952 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.020979 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.014985 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 9 | Yeast extract | 4.99501 G_PER_L | ||
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
1.998 G_PER_L |
CHEBI:17234
glucose
|
|
| 11 | Agar | 14.985 G_PER_L |
CHEBI:2509
agar
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Solutions
Trace element solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MnSO4 x n H2O | 5 G_PER_L | |
| CoCl2 x 6 H2O | 1 G_PER_L | |
| ZnSO4 x 7 H2O | 1 G_PER_L | |
| Pyridoxine hydrochloride | 0.1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.435592Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1046 |
| 2026-03-13T06:19:11.460535Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002230 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.254017+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 1046" to "jcm_medium_no_1046" for consistent matching |
| 2026-06-05T07:41:09.780448+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.178099+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-13T23:31:54.412793+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |