halomonas_cug_medium

CultureMech:002233 bacterial COMPLEX LIQUID
Original name: HALOMONAS/CUG MEDIUM

Overview

External ontology
mediadive.medium:J1050 (source link unavailable) — HALOMONAS/CUG MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1050

Composition

flowchart LR
    CultureMech_002233["halomonas_cug_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002233 --> ing0_Yeast_extract
    ing1_Peptone["Peptone"]
    CultureMech_002233 --> ing1_Peptone
    ing2_Glucose["Glucose"]
    CultureMech_002233 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Casamino_acids["Casamino acids"]
    CultureMech_002233 --> ing3_Casamino_acids
    ing4_Starch["Starch"]
    CultureMech_002233 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002233 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_NaCl["NaCl"]
    CultureMech_002233 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Na2SO4["Na2SO4"]
    CultureMech_002233 --> ing7_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing7_Na2SO4 -.-> CHEBI_32149
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002233 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_KCl["KCl"]
    CultureMech_002233 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002233 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_K2HPO4["K2HPO4"]
    CultureMech_002233 --> ing11_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing11_K2HPO4 -.-> CHEBI_131527
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002233 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13_NaNO3["NaNO3"]
    CultureMech_002233 --> ing13_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing13_NaNO3 -.-> CHEBI_63005

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L
2 Peptone 0.5 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
4 Casamino acids 0.5 G_PER_L
5 Starch 0.5 G_PER_L CHEBI:28017
starch
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
7 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
8 Na2SO4
2Na.O4S 142.043 g/mol
20.1 G_PER_L CHEBI:32149
sodium sulfate
9 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
10 KCl
Cl.K 74.551 g/mol
1 G_PER_L CHEBI:32588
potassium chloride
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
13 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
14 NaNO3
NO3.Na 84.994 g/mol
0.05 G_PER_L CHEBI:63005
sodium nitrate

Preparation

  1. Mix components thoroughly and adjust pH to 7.5. For preparation of solid medium, add 15.0 g/L agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.447724Z mediadive-import Imported from MediaDive Source: JCM, ID: J1050
2026-01-28T19:48:34.675868Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:11.475370Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002233
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.270581+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOMONAS/CUG MEDIUM" to "halomonas_cug_medium" for consistent matching
2026-06-05T07:41:07.861916+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.732590+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:59.545336+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.