Overview
- External ontology
- mediadive.medium:J1051 (source link unavailable) — ENDOMICROBIUM PROAVITUM RSA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1051 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002234["endomicrobium_proavitum_rsa_medium"]
ing0_NaCl["NaCl"]
CultureMech_002234 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_002234 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002234 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002234 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_002234 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_002234 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Resazurin["Resazurin"]
CultureMech_002234 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_002234 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Glucose["Glucose"]
CultureMech_002234 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002234 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Yeast_extract["Yeast extract"]
CultureMech_002234 --> ing10_Yeast_extract
ing11_Casamino_acids["Casamino acids"]
CultureMech_002234 --> ing11_Casamino_acids
ing12_Dithiothreitol["Dithiothreitol"]
CultureMech_002234 --> ing12_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing12_Dithiothreitol -.-> CHEBI_18320
ing13_Phenyl_acetic_acid["Phenyl acetic acid"]
CultureMech_002234 --> ing13_Phenyl_acetic_acid
CHEBI_30745["CHEBI:30745"]
ing13_Phenyl_acetic_acid -.-> CHEBI_30745
ing14_Phenyl_propionic_acid["Phenyl propionic acid"]
CultureMech_002234 --> ing14_Phenyl_propionic_acid
CHEBI_28631["CHEBI:28631"]
ing14_Phenyl_propionic_acid -.-> CHEBI_28631
ing15_4_Hydroxyphenyl_acetic_acid["4-Hydroxyphenyl acetic acid"]
CultureMech_002234 --> ing15_4_Hydroxyphenyl_acetic_acid
CHEBI_18101["CHEBI:18101"]
ing15_4_Hydroxyphenyl_acetic_acid -.-> CHEBI_18101
ing16_3_Indolyl_acetic_acid["3-Indolyl acetic acid"]
CultureMech_002234 --> ing16_3_Indolyl_acetic_acid
CHEBI_16411["CHEBI:16411"]
ing16_3_Indolyl_acetic_acid -.-> CHEBI_16411
ing17_NaOH["NaOH"]
CultureMech_002234 --> ing17_NaOH
CHEBI_32145["CHEBI:32145"]
ing17_NaOH -.-> CHEBI_32145
ing18_ZnCl2["ZnCl2"]
CultureMech_002234 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002234 --> ing19_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
ing20_H3BO3["H3BO3"]
CultureMech_002234 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002234 --> ing21_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002234 --> ing22_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002234 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002234 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing25_HCl["HCl"]
CultureMech_002234 --> ing25_HCl
CHEBI_17883["CHEBI:17883"]
ing25_HCl -.-> CHEBI_17883
ing26_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002234 --> ing26_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing26_FeCl2_x_4_H2O -.-> CHEBI_86249
ing27_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002234 --> ing27_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing27_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002234 --> ing28_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing29_Valeric_acid["Valeric acid"]
CultureMech_002234 --> ing29_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing29_Valeric_acid -.-> CHEBI_17418
CultureMech_002234 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.996016 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.498008 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.398406 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0996016 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.298805 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.199203 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000398406 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 | Yeast extract | 2 G_PER_L | ||
| 12 | Casamino acids | 2 G_PER_L | ||
| 13 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
4 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 14 |
Phenyl acetic acid
C8H8O2 136.15 g/mol
|
0.68 G_PER_L |
CHEBI:30745
phenylacetic acid
|
|
| 15 |
Phenyl propionic acid
C9H10O2 150.177 g/mol
|
0.75 G_PER_L |
CHEBI:28631
3-phenylpropionic acid
|
|
| 16 |
4-Hydroxyphenyl acetic acid
C8H8O3 152.149 g/mol
|
0.76 G_PER_L |
CHEBI:18101
4-hydroxyphenylacetic acid
|
|
| 17 |
3-Indolyl acetic acid
C10H9NO2 175.187 g/mol
|
0.88 G_PER_L |
CHEBI:16411
indole-3-acetic acid
|
|
| 18 |
NaOH
HO.Na 39.997 g/mol
|
100.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
[Merged 2 duplicates: 100.0, 0.4] |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 20 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 23 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 26 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 27 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 28 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 29 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 30 |
Valeric acid
C5H10O2 102.133 g/mol
|
25 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 31 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
25 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 32 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 33 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 34 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 35 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
25 G_PER_L |
CHEBI:16135
isobutyric acid
|
Preparation
- Mix components thoroughly and autoclave. Cool medium under a N2-CO2 (4:1, v/v) gas stream, and add the following solutions (autoclaved or *filter-sterilized) anaerobically and aseptically:
- Adjust pH to 7.1 - 7.2. Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (filter-sterilized):
- Autoclave and store under a N2 atmosphere.
- Adjust pH to 7.5 with concentrated NaOH solution.
Solutions
Trace vitamins solution (see Medium No. 284)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:18.450518Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1051 |
| 2026-01-28T19:48:31.102181Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:51.756122+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:23.123519Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:11.488160Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002234 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.285004+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ENDOMICROBIUM PROAVITUM RSA MEDIUM" to "endomicrobium_proavitum_rsa_medium" for consistent matching |
| 2026-05-16T09:03:31.461936+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:59.555103+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:38.110248+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:56.703102+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |