maize_meal_sardine_agar

CultureMech:002237 bacterial COMPLEX SOLID_AGAR
Original name: MAIZE MEAL-SARDINE AGAR

Overview

External ontology
mediadive.medium:J1054 (source link unavailable) — MAIZE MEAL-SARDINE AGAR
Applications
  • Microbial cultivation
Notes
JCM Medium J1054 Maize Meal-Sardine Agar lists 20 g maize meal, 20 g small dried sardine, 20 g agar, and 1 L distilled water. JCM instructs boiling the maize meal and sardine with 500 ml distilled water for 20 min, filtering through cloth, bringing the filtrate to 1 L, adding agar, and boiling until dissolved; JCM's standing instruction is to autoclave media at 121 C for 15 min unless otherwise stated.

Composition

flowchart LR
    CultureMech_002237["maize_meal_sardine_agar"]
    ing0_Maize_meal["Maize meal"]
    CultureMech_002237 --> ing0_Maize_meal
    ing1_Small_dried_sardine["Small dried sardine"]
    CultureMech_002237 --> ing1_Small_dried_sardine
    ing2_Agar["Agar"]
    CultureMech_002237 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_002237 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Maize meal 20 G_PER_L JCM Medium 1054 lists 20 g/L maize meal, extracted by boiling with small dried sardine in 500 ml of the final distilled-water volume.
2 Small dried sardine 20 G_PER_L JCM Medium 1054 lists 20 g/L small dried sardine, extracted by boiling with maize meal in 500 ml of the final distilled-water volume.
3 Agar 20 G_PER_L CHEBI:2509
agar
 JCM Medium 1054 lists 20 g/L agar.
4 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 JCM Medium 1054 lists 1 L distilled water.

Preparation

  1. Boil 20 g maize meal and 20 g small dried sardine with 500 ml distilled water for 20 min.
  2. Filter the maize-sardine extract through cloth.
  3. Bring the filtrate to 1 L with distilled water.
  4. Add 20 g agar and boil until dissolved.
  5. Autoclave the completed medium at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.462234Z mediadive-import Imported from MediaDive Source: JCM, ID: J1054
2026-03-13T06:19:11.513473Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002237
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.323849+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MAIZE MEAL-SARDINE AGAR" to "maize_meal_sardine_agar" for consistent matching
2026-06-05T07:41:12.595137+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_jcm_j1054_maize_sardine_score15.py RESOLVED_JCM_J1054_MAIZE_SARDINE_SCORE15 JCM Medium J1054 Maize Meal-Sardine Agar lists 20 g maize meal, 20 g small dried sardine, 20 g agar, and 1 L distilled water. JCM instructs boiling the maize meal and sardine with 500 ml distilled water for 20 min, filtering through cloth, bringing the filtrate to 1 L, adding agar, and boiling until dissolved; JCM's standing instruction is to autoclave media at 121 C for 15 min unless otherwise stated. Restored the 1 L distilled-water row from the source, grounded water and agar, and split the importer's single misclassified preparation note into explicit heat, filter, mix, heat, and autoclave steps.