db_characterization_medium_no_2_with_1_sea_salts

CultureMech:002239 bacterial COMPLEX LIQUID
Original name: DB CHARACTERIZATION MEDIUM NO.2 WITH 1% SEA SALTS

Overview

External ontology
mediadive.medium:J1058 (source link unavailable) — DB CHARACTERIZATION MEDIUM NO.2 WITH 1% SEA SALTS
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1058

Composition

flowchart LR
    CultureMech_002239["db_characterization_medium_no_2_with_1_sea_salts"]
    ing0_Peptone["Peptone"]
    CultureMech_002239 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002239 --> ing1_Yeast_extract
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002239 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002239 --> ing3_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing3_Sodium_pyruvate -.-> CHEBI_50144
    ing4_NaCl["NaCl"]
    CultureMech_002239 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002239 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002239 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_KCl["KCl"]
    CultureMech_002239 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_CaCl2["CaCl2"]
    CultureMech_002239 --> ing8_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing8_CaCl2 -.-> CHEBI_3312
    ing9_K2HPO4["K2HPO4"]
    CultureMech_002239 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_KH2PO4["KH2PO4"]
    CultureMech_002239 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_Biotin["Biotin"]
    CultureMech_002239 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_002239 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002239 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002239 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Riboflavin["Riboflavin"]
    CultureMech_002239 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002239 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002239 --> ing17_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_002239 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002239 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Lipoic_acid["Lipoic acid"]
    CultureMech_002239 --> ing20_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing20_Lipoic_acid -.-> CHEBI_16494
    ing21_HCl["HCl"]
    CultureMech_002239 --> ing21_HCl
    CHEBI_17883["CHEBI:17883"]
    ing21_HCl -.-> CHEBI_17883
    ing22_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002239 --> ing22_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing22_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing23_ZnCl2["ZnCl2"]
    CultureMech_002239 --> ing23_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing23_ZnCl2 -.-> CHEBI_49976
    ing24_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002239 --> ing24_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing24_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing25_H3BO3["H3BO3"]
    CultureMech_002239 --> ing25_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing25_H3BO3 -.-> CHEBI_33118
    ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002239 --> ing26_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002239 --> ing27_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002239 --> ing28_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002239 --> ing29_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Peptone 0.25 G_PER_L Copied from referenced JCM Medium 574
2 Yeast extract 0.05 G_PER_L Copied from referenced JCM Medium 574
3 NH4Cl
Cl.H4N 53.492 g/mol
0.26745 G_PER_L CHEBI:31206
ammonium chloride
 Copied from referenced JCM Medium 574
4 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1.1004 G_PER_L CHEBI:50144
sodium pyruvate
 Copied from referenced JCM Medium 574
5 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
 Copied from referenced JCM Medium 574
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
30 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Copied from referenced JCM Medium 574
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
35 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Copied from referenced JCM Medium 574
8 KCl
Cl.K 74.551 g/mol
7 G_PER_L CHEBI:32588
potassium chloride
 Copied from referenced JCM Medium 574
9 CaCl2
Ca.2Cl 110.984 g/mol
0.5549 G_PER_L CHEBI:3312
calcium dichloride
 Copied from referenced JCM Medium 574
10 K2HPO4
HO4P.2K 174.174 g/mol
14.5263 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Copied from referenced JCM Medium 574
11 KH2PO4
H2O4P.K 136.084 g/mol
2.25901 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Copied from referenced JCM Medium 574
12 Biotin
C10H16N2O3S 244.316 g/mol
0.003 G_PER_L CHEBI:15956
biotin
 Copied from referenced JCM Medium 574
13 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
 Copied from referenced JCM Medium 574
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.05 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Copied from referenced JCM Medium 574
15 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.033 G_PER_L CHEBI:49105
thiamine hydrochloride
 Copied from referenced JCM Medium 574
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
 Copied from referenced JCM Medium 574
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.033 G_PER_L CHEBI:15940
nicotinic acid
 Copied from referenced JCM Medium 574
18 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.017 G_PER_L CHEBI:31345
Calcium pantothenate
 Copied from referenced JCM Medium 574
19 Vitamin B12 0.017 G_PER_L CHEBI:176843
vitamin B12
 Copied from referenced JCM Medium 574
20 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.013 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Copied from referenced JCM Medium 574
21 Lipoic acid
C8H14O2S2 206.332 g/mol
0.01 G_PER_L CHEBI:16494
lipoic acid
 Copied from referenced JCM Medium 574
22 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
 Copied from referenced JCM Medium 574
23 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 Copied from referenced JCM Medium 574
24 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
 Copied from referenced JCM Medium 574
25 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Copied from referenced JCM Medium 574
26 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
 Copied from referenced JCM Medium 574
27 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
 Copied from referenced JCM Medium 574
28 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 Copied from referenced JCM Medium 574
29 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
 Copied from referenced JCM Medium 574
30 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Copied from referenced JCM Medium 574

Preparation

  1. Use Medium No. 574 supplemented with 10.0 g/L sea salts (Sigma).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.475359Z mediadive-import Imported from MediaDive Source: JCM, ID: J1058
2026-02-03T11:26:52.753718 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:11.530856Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002239
2026-03-17T19:45:24.349039+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DB CHARACTERIZATION MEDIUM NO.2 WITH 1% SEA SALTS" to "db_characterization_medium_no_2_with_1_sea_salts" for consistent matching
2026-04-04T18:49:19.826917 copy_referenced_compositions RESOLVED_REFERENCE Resolved reference by copying from db_characterization_medium_no_2 (CultureMech:002922)
2026-05-16T08:57:27.165536+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(1 entries)
2026-06-05T07:40:55.327932+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.122305+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.