halobacteria_mh4_2_medium

CultureMech:002242 archaea COMPLEX LIQUID
Original name: HALOBACTERIA MH4.2 MEDIUM

Overview

External ontology
mediadive.medium:J1061 (source link unavailable) — HALOBACTERIA MH4.2 MEDIUM
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1061

Composition

flowchart LR
    CultureMech_002242["halobacteria_mh4_2_medium"]
    ing0_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002242 --> ing0_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing0_Sodium_pyruvate -.-> CHEBI_50144
    ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_002242 --> ing1_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002242 --> ing2_Yeast_extract
    ing3_Tryptone["Tryptone"]
    CultureMech_002242 --> ing3_Tryptone
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002242 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002242 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaCl["NaCl"]
    CultureMech_002242 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002242 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_K2SO4["K2SO4"]
    CultureMech_002242 --> ing8_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing8_K2SO4 -.-> CHEBI_32036
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002242 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002242 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_Tris_HCl["Tris-HCl"]
    CultureMech_002242 --> ing11_Tris_HCl
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002242 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_H3BO3["H3BO3"]
    CultureMech_002242 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002242 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002242 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002242 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002242 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
9.90099 G_PER_L CHEBI:50144
sodium pyruvate
2 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
9.90099 G_PER_L CHEBI:53258
sodium citrate
3 Yeast extract 19.802 G_PER_L
4 Tryptone 19.802 G_PER_L
5 K2HPO4
HO4P.2K 174.174 g/mol
5.94059 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NH4Cl
Cl.H4N 53.492 g/mol
5.94059 G_PER_L CHEBI:31206
ammonium chloride
7 NaCl
Cl.Na 58.443 g/mol
1980.2 G_PER_L CHEBI:26710
sodium chloride
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
4.9505 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 K2SO4
2K.O4S 174.259 g/mol
49.505 G_PER_L CHEBI:32036
potassium sulfate
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.356436 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03356436 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.00356436, 0.03]
12 Tris-HCl 100 G_PER_L
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g agar.
  2. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.483332Z mediadive-import Imported from MediaDive Source: JCM, ID: J1061
2026-01-28T19:48:27.267412Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:36.448007Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:15.568438Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:11.542964Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002242
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.363172+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOBACTERIA MH4.2 MEDIUM" to "halobacteria_mh4_2_medium" for consistent matching
2026-06-05T07:41:07.561299+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.453484+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.