m25y_medium

CultureMech:002246 bacterial COMPLEX LIQUID
Original name: M25Y MEDIUM

Overview

External ontology
mediadive.medium:J1066 (source link unavailable) — M25Y MEDIUM
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1066

Composition

flowchart LR
    CultureMech_002246["m25y_medium"]
    ing0_MES["MES"]
    CultureMech_002246 --> ing0_MES
    CHEBI_39010["CHEBI:39010"]
    ing0_MES -.-> CHEBI_39010
    ing1_NaCl["NaCl"]
    CultureMech_002246 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002246 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002246 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002246 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KCl["KCl"]
    CultureMech_002246 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002246 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_K2HPO4["K2HPO4"]
    CultureMech_002246 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002246 --> ing8_Yeast_extract
    ing9_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002246 --> ing9_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing9_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing10_Resazurin["Resazurin"]
    CultureMech_002246 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_002246 --> ing11_Trace_vitamins__see_Medium_No__197_
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002246 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Na2_EDTA["Na2-EDTA"]
    CultureMech_002246 --> ing13_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing13_Na2_EDTA -.-> CHEBI_64734
    ing14_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
    CultureMech_002246 --> ing14_NiSO4_x_6_H2O
    CHEBI_53001["CHEBI:53001"]
    ing14_NiSO4_x_6_H2O -.-> CHEBI_53001
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002246 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_H3BO3["H3BO3"]
    CultureMech_002246 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002246 --> ing17_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002246 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002246 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_Na2SeO3["Na2SeO3"]
    CultureMech_002246 --> ing20_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing20_Na2SeO3 -.-> CHEBI_48843

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 MES 3.81605 G_PER_L CHEBI:39010
MES
2 NaCl
Cl.Na 58.443 g/mol
24.4618 G_PER_L CHEBI:26710
sodium chloride
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.64188 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.20744 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.244618 G_PER_L CHEBI:31206
ammonium chloride
6 KCl
Cl.K 74.551 g/mol
0.332681 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.136986 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 K2HPO4
HO4P.2K 174.174 g/mol
0.136986 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
9 Yeast extract 0.195695 G_PER_L
10 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
1 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
11 Resazurin
C12H7NO4 229.191 g/mol
0.000978474 G_PER_L CHEBI:8806
Resazurin
12 Trace vitamins (see Medium No. 197) 10 G_PER_L
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
29 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
15 NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
0.11 G_PER_L CHEBI:53001
nickel sulfate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
18 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.1 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 Na2SeO3
2Na.O3Se 172.937 g/mol
0.05 G_PER_L CHEBI:48843
disodium selenite

Preparation

  1. Adjust pH to 5.5. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter sterilized):
  2. First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.

Solutions

Trace elements

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
MnSO4 x n H2O 3.26 G_PER_L
CoCl2 x 6 H2O 1.8 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.502866Z mediadive-import Imported from MediaDive Source: JCM, ID: J1066
2026-01-28T19:48:21.223038Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:11.592689Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002246
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.424798+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M25Y MEDIUM" to "m25y_medium" for consistent matching
2026-06-05T07:41:11.786635+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.676596+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.426874+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace elements' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).