freshwater_iron_oxidizing_bacteria_medium_ii

Overview

External ontology

mediadive.medium:J1067 (source link unavailable) — FRESHWATER IRON-OXIDIZING BACTERIA MEDIUM-II

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1067

Composition

flowchart LR
    CultureMech_002247["freshwater_iron_oxidizing_bacteria_medium_ii"]
    ing0_Agar["Agar"]
    CultureMech_002247 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_NaHCO3["NaHCO3"]
    CultureMech_002247 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002247 --> ing2_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing2_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002247 --> ing3_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_x_9_H2O -.-> CHEBI_76209
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002247 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002247 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002247 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_KH2PO4["KH2PO4"]
    CultureMech_002247 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002247 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002247 --> ing9_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing10_NaCl["NaCl"]
    CultureMech_002247 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710
    ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002247 --> ing11_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002247 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002247 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002247 --> ing14_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing14_AlK_SO4_2 -.-> CHEBI_86463
    ing15_H3BO3["H3BO3"]
    CultureMech_002247 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002247 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Biotin["Biotin"]
    CultureMech_002247 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_002247 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002247 --> ing19_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing20_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002247 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Riboflavin["Riboflavin"]
    CultureMech_002247 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002247 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002247 --> ing23_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_002247 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002247 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26_Lipoic_acid["Lipoic acid"]
    CultureMech_002247 --> ing26_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26_Lipoic_acid -.-> CHEBI_16494

Ingredients (27)

Preparation

1. Bottom layer:
2. Top layer:
3. Autoclave both layer solutions. Shortly after autoclaving, pipette the bottom layer into each culture vessel (typically 5 ml in 60 ml glass vials, use pipette tips that have been cut to enlarge the opening). The bottom layer is allowed to cool for at least 30 min to ensure its solidification.
4. After cooling of the top layer solution, add per liter the following solutions (filter-sterilized):
5. Add the top layer solution in 5 to 6 times the volume of the bottom layer to the culture vessels containing the bottom layer (e.g., 25 to 30 ml in 60 ml glass vials with 5 ml of the bottom layer).
6. Then, cap the culture vessels with silicone rubber stoppers, and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture.
7. Comment: The medium can be stored in culture vessels for a certain time by capping with butyl rubber stoppers, instead of silicon rubber stoppers and keeping anoxic in the vessels. Silicone rubber stopper is O2 permeable and creates a mircoaerophilic condition in the culture vessels.
8. Prewarm distilled water at 50C in a beaker with a stir bar. While rapidly starring the water, add FeSO4 x 7H2O, followed immediately by Na2S x 9H2O. A thick black precipitate will form instantly. Stir continuously for 2 to 3 min. Remove the mixture to a narrow-mouthed glass bottle (500 ml) that can be tightly stopped. The bottle is filled to the top with distilled water and capped. Allow to settle for several hours, decant and replace the overlaying water. Repeat this procedure at least five times to wash the FeS precipitate. Check pH to be close to neutral. To stock the FeS solution, fill the bottle with distilled water and cap. Remove the overlaying water when prepare the bottom layer.
9. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance