Overview
- External ontology
- mediadive.medium:J106 (source link unavailable) — HALF STRENGTH SKIM MILK AGAR
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- JCM Medium 106 defines Half Strength Skim Milk Agar as Solution A with 25.0 g Skim milk (BD-Difco) and 500.0 ml distilled water, adjusted to pH 7.0, plus Solution B with 20.0 g agar and 500.0 ml distilled water. Solutions A and B are autoclaved separately and mixed aseptically.
Ingredients (0)
No ingredients recorded.
Preparation
- Adjust Solution A to pH 7.0.
- Autoclave Solutions A and B separately and mix aseptically.
Solutions
Solution A
Dose: 500.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Skim milk (BD-Difco) | 50.0 G_PER_L | JCM Medium 106 lists 25.0 g Skim milk (BD-Difco) in the 500 ml Solution A stock; this complex milk product is retained as an opaque component. |
| Distilled water | 1.0 L | JCM Medium 106 lists 500 ml distilled water in this stock. |
JCM Medium 106 adds 500.0 ml/L Solution A.
Solution B
Dose: 500.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Agar | 40.0 G_PER_L | JCM Medium 106 lists 20.0 g agar in the 500 ml Solution B stock. |
| Distilled water | 1.0 L | JCM Medium 106 lists 500 ml distilled water in this stock. |
JCM Medium 106 adds 500.0 ml/L Solution B.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.739729Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J106 |
| 2026-03-13T06:19:11.641583Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002250 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.482774+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALF STRENGTH SKIM MILK AGAR" to "half_strength_skim_milk_agar" for consistent matching |
| 2026-06-05T07:41:07.195634+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_jcm_j106_skim_milk_agar_score15.py | RESOLVED_JCM_J106_SCORE15 | JCM Medium 106 defines Half Strength Skim Milk Agar as Solution A with 25.0 g Skim milk (BD-Difco) and 500.0 ml distilled water, adjusted to pH 7.0, plus Solution B with 20.0 g agar and 500.0 ml distilled water. Solutions A and B are autoclaved separately and mixed aseptically. Replaced the imported stock-solution concentrations with explicit nested Solution A and Solution B recipes. |