Overview
- External ontology
- mediadive.medium:J1075 (source link unavailable) — CUNICULIPLASMA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 1.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1075
Composition
flowchart LR
CultureMech_002255["cuniculiplasma_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002255 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_002255 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002255 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002255 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002255 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_Beef_extract["Beef extract"]
CultureMech_002255 --> ing5_Beef_extract
ing6_Yeast_extract["Yeast extract"]
CultureMech_002255 --> ing6_Yeast_extract
ing7_Tryptone["Tryptone"]
CultureMech_002255 --> ing7_Tryptone
ing8_Betaine["Betaine"]
CultureMech_002255 --> ing8_Betaine
CHEBI_17750["CHEBI:17750"]
ing8_Betaine -.-> CHEBI_17750
ing9_ZnCl2["ZnCl2"]
CultureMech_002255 --> ing9_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing9_ZnCl2 -.-> CHEBI_49976
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002255 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_002255 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002255 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002255 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002255 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002255 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.28586 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.276954 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.24728 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0692384 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.97824e-05 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 | Beef extract | 0.98912 G_PER_L | ||
| 7 | Yeast extract | 0.098912 G_PER_L | ||
| 8 | Tryptone | 0.98912 G_PER_L | ||
| 9 |
Betaine
C5H11NO2 117.148 g/mol
|
0.593472 G_PER_L |
CHEBI:17750
glycine betaine
|
|
| 10 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 1.0 - 1.2 with H2SO4.
Solutions
Kao and Michayluk vitamin solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.544782Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1075 |
| 2026-03-09T08:04:32.026368+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:11.694631Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002255 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.547493+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CUNICULIPLASMA MEDIUM" to "cuniculiplasma_medium" for consistent matching |
| 2026-05-16T09:03:28.392670+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:55.006112+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.036463+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |