Overview
- External ontology
- mediadive.medium:J1078 (source link unavailable) — ALKALIPHILUS 3B MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1078
Composition
flowchart LR
CultureMech_002258["alkaliphilus_3b_medium"]
ing0_NaCl["NaCl"]
CultureMech_002258 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NH4Cl["NH4Cl"]
CultureMech_002258 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KH2PO4["KH2PO4"]
CultureMech_002258 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_002258 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KCl["KCl"]
CultureMech_002258 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002258 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Tris_base["Tris base"]
CultureMech_002258 --> ing6_Tris_base
CHEBI_9754["CHEBI:9754"]
ing6_Tris_base -.-> CHEBI_9754
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002258 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing8_Resazurin["Resazurin"]
CultureMech_002258 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Yeast_extract["Yeast extract"]
CultureMech_002258 --> ing9_Yeast_extract
ing10_Crotonic_acid["Crotonic acid"]
CultureMech_002258 --> ing10_Crotonic_acid
CHEBI_41131["CHEBI:41131"]
ing10_Crotonic_acid -.-> CHEBI_41131
ing11_Na2CO3["Na2CO3"]
CultureMech_002258 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002258 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_HCl["HCl"]
CultureMech_002258 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002258 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_H3BO3["H3BO3"]
CultureMech_002258 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002258 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002258 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002258 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002258 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002258 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002258 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.993049 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.993049 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0993049 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.0993049 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.0993049 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0993049 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
Tris base
C4H11NO3 121.136 g/mol
|
2.97915 G_PER_L |
CHEBI:9754
tris
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.48957 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000993049 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 | Yeast extract | 50 G_PER_L | ||
| 11 |
Crotonic acid
C4H6O2 86.09 g/mol
|
20 G_PER_L |
CHEBI:41131
crotonic acid
|
|
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
20 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
HCl
HCl 36.461 g/mol
|
12.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere):
- Check final pH be around 8.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.555223Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1078 |
| 2026-03-13T06:19:11.723649Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002258 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.581731+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIPHILUS 3B MEDIUM" to "alkaliphilus_3b_medium" for consistent matching |
| 2026-06-05T07:40:48.070111+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.861485+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |