Overview
- External ontology
- mediadive.medium:J1080 (source link unavailable) — HALODESULFURIARCHAEUM MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1080
Composition
flowchart LR
CultureMech_002260["halodesulfuriarchaeum_medium"]
ing0_NaCl["NaCl"]
CultureMech_002260 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_002260 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002260 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_HEPES["HEPES"]
CultureMech_002260 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_MgCl2["MgCl2"]
CultureMech_002260 --> ing4_MgCl2
CHEBI_6636["CHEBI:6636"]
ing4_MgCl2 -.-> CHEBI_6636
ing5_NaHCO3["NaHCO3"]
CultureMech_002260 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002260 --> ing6_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing6_Sodium_pyruvate -.-> CHEBI_50144
ing7_Yeast_extract["Yeast extract"]
CultureMech_002260 --> ing7_Yeast_extract
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002260 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_EDTA["EDTA"]
CultureMech_002260 --> ing9_EDTA
CHEBI_4735["CHEBI:4735"]
ing9_EDTA -.-> CHEBI_4735
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002260 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002260 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002260 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_002260 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002260 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002260 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002260 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002260 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_NaOH["NaOH"]
CultureMech_002260 --> ing18_NaOH
CHEBI_32145["CHEBI:32145"]
ing18_NaOH -.-> CHEBI_32145
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002260 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002260 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
8571.43 G_PER_L |
CHEBI:26710
sodium chloride
|
Copied from referenced JCM Medium 1079 |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
89.2857 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Copied from referenced JCM Medium 1079 |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
17.8571 G_PER_L |
CHEBI:31206
ammonium chloride
|
Copied from referenced JCM Medium 1079 |
| 4 | HEPES | 250 G_PER_L |
CHEBI:46756
HEPES
|
Copied from referenced JCM Medium 1079 |
| 5 |
MgCl2
2Cl.Mg 95.211 g/mol
|
1 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Copied from referenced JCM Medium 1079 |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Copied from referenced JCM Medium 1079 |
| 7 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
10 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Copied from referenced JCM Medium 1079 |
| 8 | Yeast extract | 0.2 G_PER_L | Copied from referenced JCM Medium 1079 | |
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Copied from referenced JCM Medium 1079 |
| 10 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Copied from referenced JCM Medium 1079 |
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 1079 |
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 1079 |
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Copied from referenced JCM Medium 1079 |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Copied from referenced JCM Medium 1079 |
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Copied from referenced JCM Medium 1079 |
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Copied from referenced JCM Medium 1079 |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Copied from referenced JCM Medium 1079 |
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 1079 |
| 19 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Copied from referenced JCM Medium 1079 |
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Copied from referenced JCM Medium 1079 |
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Copied from referenced JCM Medium 1079 |
Preparation
- Use Medium No. 1079 amended by the replacement of sodium pyruvate with final 50 mM sodium formate and the supplement with final 200 mg/L yeast extract.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.562619Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1080 |
| 2026-02-03T11:26:52.859745 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:11.739226Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002260 |
| 2026-03-17T19:45:24.599109+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALODESULFURIARCHAEUM MEDIUM" to "halodesulfuriarchaeum_medium" for consistent matching |
| 2026-04-04T18:49:19.057066 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from halanaeroarchaeum_medium (CultureMech:002259) |
| 2026-05-16T08:57:04.109357+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:16.508276+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.721371+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |