desulfurella_tr1_medium

CultureMech:002263 bacterial COMPLEX LIQUID
Original name: DESULFURELLA TR1 MEDIUM

Overview

External ontology
mediadive.medium:J1083 (source link unavailable) — DESULFURELLA TR1 MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1083

Composition

flowchart LR
    CultureMech_002263["desulfurella_tr1_medium"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002263 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002263 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002263 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_002263 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002263 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002263 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
    CultureMech_002263 --> ing6_Sodium_acetate_x_3_H2O
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002263 --> ing7_Yeast_extract
    ing8_Sulfur["Sulfur"]
    CultureMech_002263 --> ing8_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing8_Sulfur -.-> CHEBI_26833
    ing9_Resazurin["Resazurin"]
    CultureMech_002263 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_NaHCO3["NaHCO3"]
    CultureMech_002263 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002263 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_HCl["HCl"]
    CultureMech_002263 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_H3BO3["H3BO3"]
    CultureMech_002263 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002263 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002263 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002263 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002263 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_ZnCl2["ZnCl2"]
    CultureMech_002263 --> ing18_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing18_ZnCl2 -.-> CHEBI_49976
    ing19_NaOH["NaOH"]
    CultureMech_002263 --> ing19_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing19_NaOH -.-> CHEBI_32145
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002263 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002263 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002263 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_Biotin["Biotin"]
    CultureMech_002263 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002263 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002263 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002263 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002263 --> ing27_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002263 --> ing28_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing28_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_002263 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843
    CultureMech_002263 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.519099 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.401567 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.29383 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
0.29383 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0979432 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.107738 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
0.666014 G_PER_L CHEBI:32954
sodium acetate
8 Yeast extract 0.0979432 G_PER_L
9 Sulfur
S 32.067 g/mol
0.783546 G_PER_L CHEBI:26833
sulfur atom
10 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
11 NaHCO3
CHO3.Na 84.006 g/mol
12.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 HCl
HCl 36.461 g/mol
10 G_PER_L CHEBI:17883
hydrogen chloride
14 H3BO3
H3BO3 61.833 g/mol
0.0611881 G_PER_L CHEBI:33118
boric acid
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0606436 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.934158 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.0638614 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0127327 G_PER_L CHEBI:34887
nickel dichloride
19 ZnCl2
2Cl.Zn 136.296 g/mol
0.0670297 G_PER_L CHEBI:49976
zinc dichloride
20 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0173 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0294 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0205 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
26 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.1 G_PER_L CHEBI:30753
4-aminobenzoic acid
27 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.2 G_PER_L CHEBI:49105
thiamine hydrochloride
28 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
29 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride
30 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
31 Riboflavin
C17H20N4O6 376.369 g/mol
0.1 G_PER_L CHEBI:17015
riboflavin

Preparation

  1. Mix components, except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):
  2. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium and sulfur into culture vessels (e.g., 50 ml of the medium and 0.04 g of sulfur in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, anaerobically add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
  3. Adjust pH to 6.0, if necessary.
  4. Filter-sterilize and store under a N2 atmosphere.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.570070Z mediadive-import Imported from MediaDive Source: JCM, ID: J1083
2026-03-13T06:19:11.765706Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002263
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.627823+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFURELLA TR1 MEDIUM" to "desulfurella_tr1_medium" for consistent matching
2026-06-05T07:40:58.181007+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.180512+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.