anaerobacter_medium

CultureMech:002265 bacterial COMPLEX LIQUID
Original name: ANAEROBACTER MEDIUM

Overview

External ontology
mediadive.medium:J1085 (source link unavailable) — ANAEROBACTER MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1085

Composition

flowchart LR
    CultureMech_002265["anaerobacter_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002265 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002265 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KCl["KCl"]
    CultureMech_002265 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002265 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002265 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002265 --> ing5_Yeast_extract
    ing6_Resazurin["Resazurin"]
    CultureMech_002265 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Sucrose["Sucrose"]
    CultureMech_002265 --> ing7_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing7_Sucrose -.-> CHEBI_17992
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002265 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002265 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002265 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_HCl["HCl"]
    CultureMech_002265 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002265 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_ZnCl2["ZnCl2"]
    CultureMech_002265 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002265 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_002265 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002265 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002265 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002265 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002265 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.316699 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.316699 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
0.316699 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.316699 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.316699 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Yeast extract 0.959693 G_PER_L
7 Resazurin
C12H7NO4 229.191 g/mol
0.000959693 G_PER_L CHEBI:8806
Resazurin
8 Sucrose
C12H22O11 342.297 g/mol
10 G_PER_L CHEBI:17992
sucrose
9 NaHCO3
CHO3.Na 84.006 g/mol
18.3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following sterile solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere or *filter-sterilized and stored under 80% N2 and 20% CO2 gas mixture):
  2. Dispense the medium into culture vessels under a N2 atmosphere and seal with butyl rubber stoppers. Finally, add per litter the following solutions (autoclaved and stored under a N2 atmosphere):

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.579186Z mediadive-import Imported from MediaDive Source: JCM, ID: J1085
2026-03-13T06:19:11.786929Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002265
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.652439+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANAEROBACTER MEDIUM" to "anaerobacter_medium" for consistent matching
2026-06-05T07:40:16.520892+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.726836+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.