natronolimnobius_aht32_medium_b

CultureMech:002272 archaea COMPLEX LIQUID
Original name: NATRONOLIMNOBIUS AHT32 MEDIUM (B)

Overview

External ontology
mediadive.medium:J1092 (source link unavailable) — NATRONOLIMNOBIUS AHT32 MEDIUM (B)
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1092

Composition

flowchart LR
    CultureMech_002272["natronolimnobius_aht32_medium_b"]
    ing0_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_002272 --> ing0_Trace_vitamins__see_Medium_No__197_
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002272 --> ing1_Yeast_extract
    ing2_Sodium_butyrate["Sodium butyrate"]
    CultureMech_002272 --> ing2_Sodium_butyrate
    CHEBI_64103["CHEBI:64103"]
    ing2_Sodium_butyrate -.-> CHEBI_64103
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_002272 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_Na2CO3["Na2CO3"]
    CultureMech_002272 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002272 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_NaCl["NaCl"]
    CultureMech_002272 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_K2HPO4["K2HPO4"]
    CultureMech_002272 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_KCl["KCl"]
    CultureMech_002272 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NH4Cl["NH4Cl"]
    CultureMech_002272 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_MgCl2["MgCl2"]
    CultureMech_002272 --> ing10_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing10_MgCl2 -.-> CHEBI_6636

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Trace vitamins (see Medium No. 197) 10 G_PER_L
2 Yeast extract 0.2 G_PER_L
3 Sodium butyrate
C4H7O2.Na 110.088 g/mol
20 G_PER_L CHEBI:64103
sodium butyrate
4 Sodium acetate
C2H3O2.Na 82.034 g/mol
20 G_PER_L CHEBI:32954
sodium acetate
5 Na2CO3
CO3.2Na 105.988 g/mol
46250 G_PER_L CHEBI:29377
sodium carbonate
6 NaHCO3
CHO3.Na 84.006 g/mol
8750 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 NaCl
Cl.Na 58.443 g/mol
4000 G_PER_L CHEBI:26710
sodium chloride
8 K2HPO4
HO4P.2K 174.174 g/mol
250 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
9 KCl
Cl.K 74.551 g/mol
1250 G_PER_L CHEBI:32588
potassium chloride
10 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
11 MgCl2
2Cl.Mg 95.211 g/mol
1 G_PER_L CHEBI:6636
magnesium dichloride

Preparation

  1. Aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
  2. Distribute the medium into culture vessels, replace the gas phase with N2 containing 5% O2 (by vol.), and seal with butyl rubber stoppers.
  3. Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.599532Z mediadive-import Imported from MediaDive Source: JCM, ID: J1092
2026-03-13T06:19:11.836163Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002272
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.710290+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONOLIMNOBIUS AHT32 MEDIUM (B)" to "natronolimnobius_aht32_medium_b" for consistent matching
2026-06-05T07:41:39.397699+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.451628+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.