seawater_pyruvate_thiosulfate_medium

CultureMech:002276 bacterial COMPLEX LIQUID
Original name: SEAWATER PYRUVATE THIOSULFATE MEDIUM

Overview

External ontology
mediadive.medium:J1097 (source link unavailable) — SEAWATER PYRUVATE THIOSULFATE MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1097

Composition

flowchart LR
    CultureMech_002276["seawater_pyruvate_thiosulfate_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002276 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002276 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002276 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002276 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_002276 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_KCl["KCl"]
    CultureMech_002276 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Resazurin["Resazurin"]
    CultureMech_002276 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002276 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002276 --> ing8_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing8_Sodium_pyruvate -.-> CHEBI_50144
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_002276 --> ing9_Yeast_extract
    ing10_Coenzyme_M["Coenzyme M"]
    CultureMech_002276 --> ing10_Coenzyme_M
    CHEBI_17905["CHEBI:17905"]
    ing10_Coenzyme_M -.-> CHEBI_17905
    ing11_Biotin["Biotin"]
    CultureMech_002276 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_002276 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002276 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002276 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Riboflavin["Riboflavin"]
    CultureMech_002276 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002276 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002276 --> ing17_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002276 --> ing18_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing19_Lipoic_acid["Lipoic acid"]
    CultureMech_002276 --> ing19_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing19_Lipoic_acid -.-> CHEBI_16494
    ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002276 --> ing20_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing21_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002276 --> ing21_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing21_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing22_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002276 --> ing22_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing22_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002276 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002276 --> ing24_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing24_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002276 --> ing25_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002276 --> ing26_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002276 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing28_Na2SeO4["Na2SeO4"]
    CultureMech_002276 --> ing28_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing28_Na2SeO4 -.-> CHEBI_77775
    ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002276 --> ing29_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
    CultureMech_002276 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.6078 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.94118 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.147059 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.245098 G_PER_L CHEBI:31206
ammonium chloride
5 KH2PO4
H2O4P.K 136.084 g/mol
0.196078 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 KCl
Cl.K 74.551 g/mol
0.490196 G_PER_L CHEBI:32588
potassium chloride
7 Resazurin
C12H7NO4 229.191 g/mol
0.000980392 G_PER_L CHEBI:8806
Resazurin
8 NaHCO3
CHO3.Na 84.006 g/mol
37.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
20 G_PER_L CHEBI:50144
sodium pyruvate
10 Yeast extract 10 G_PER_L
11 Coenzyme M
C2H6O3S2 142.201 g/mol
0.005 G_PER_L CHEBI:17905
coenzyme M
12 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
13 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
18 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
19 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
20 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
21 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
2 G_PER_L CHEBI:44557
nitrilotriacetic acid
22 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
1 G_PER_L CHEBI:86360
manganese(II) sulfate
23 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.8 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
24 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
25 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.2 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
26 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
27 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
28 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
29 Na2SeO4
2Na.O4Se 188.936 g/mol
0.02 G_PER_L CHEBI:77775
sodium selenate
30 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.02 G_PER_L CHEBI:63939
sodium tungstate dihydrate
31 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
12.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
32 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
12.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate

Preparation

  1. Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
  2. Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solution:
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.0 with KOH. Then proceed to add minerals.
  4. Autoclave and store under a N2 atmosphere.
  5. Comment: For strain JCM 13588, inoculate with a 20% inoculum, and incubate the vessels in inverted position.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.616160Z mediadive-import Imported from MediaDive Source: JCM, ID: J1097
2026-01-28T19:48:23.332939Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:11.881664Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002276
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.762187+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SEAWATER PYRUVATE THIOSULFATE MEDIUM" to "seawater_pyruvate_thiosulfate_medium" for consistent matching
2026-06-05T07:41:45.486298+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.816239+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:11.365668+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.