anaerolinea_medium_c

CultureMech:002281 bacterial COMPLEX LIQUID
Original name: ANAEROLINEA MEDIUM C

Overview

External ontology
mediadive.medium:J1103 (source link unavailable) — ANAEROLINEA MEDIUM C
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1103

Composition

flowchart LR
    CultureMech_002281["anaerolinea_medium_c"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002281 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002281 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002281 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002281 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002281 --> ing4_Yeast_extract
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002281 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Resazurin["Resazurin"]
    CultureMech_002281 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Glucose["Glucose"]
    CultureMech_002281 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002281 --> ing8_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_x_9_H2O -.-> CHEBI_76209
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002281 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2_EDTA["Na2-EDTA"]
    CultureMech_002281 --> ing10_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing10_Na2_EDTA -.-> CHEBI_64734
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002281 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_002281 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002281 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_002281 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002281 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002281 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002281 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002281 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NaOH["NaOH"]
    CultureMech_002281 --> ing19_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing19_NaOH -.-> CHEBI_32145
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002281 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002281 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Biotin["Biotin"]
    CultureMech_002281 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_Folic_acid["Folic acid"]
    CultureMech_002281 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002281 --> ing24_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing25_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002281 --> ing25_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing25_Thiamine_HCl -.-> CHEBI_49105
    ing26_Riboflavin["Riboflavin"]
    CultureMech_002281 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002281 --> ing27_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing27_Nicotinic_acid -.-> CHEBI_15940
    ing28_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002281 --> ing28_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing28_Calcium_pantothenate -.-> CHEBI_31345
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_002281 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843
    CultureMech_002281 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.136719 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.195312 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.146484 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.527344 G_PER_L CHEBI:31206
ammonium chloride
5 Yeast extract 2.24609 G_PER_L
6 NaHCO3
CHO3.Na 84.006 g/mol
2.44141 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Resazurin
C12H7NO4 229.191 g/mol
0.000976562 G_PER_L CHEBI:8806
Resazurin
8 Glucose
C6H12O6 180.156 g/mol
2.14844 G_PER_L CHEBI:17234
glucose
9 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
6 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
24 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
25 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
26 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
27 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
28 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
29 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
30 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
31 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
32 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Solution A:
  2. Mix components except NaHCO3 and trace vitamins, and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense Solution A into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Add trace vitamins from a sterile anaerobic stock (filter-sterilized).
  3. Solution B:
  4. Adjust pH to 7.0 and filter-sterilize. Keep under a N2 atmosphere.
  5. To complete the medium, aseptically and anaerobically add 0.1 volume of Solution B to 0.9 volume of Solution A (e.g., 18 ml Solution A in 60 ml serum bottles and 2 ml Solution B). Prior to inoculation, add per liter the following solutions:
  6. Adjust pH to 6.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.640878Z mediadive-import Imported from MediaDive Source: JCM, ID: J1103
2026-03-13T06:19:11.951882Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002281
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.840460+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANAEROLINEA MEDIUM C" to "anaerolinea_medium_c" for consistent matching
2026-06-05T07:40:49.125894+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.320908+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.