mineral_medium_with_glucose

CultureMech:002288 bacterial COMPLEX LIQUID
Original name: MINERAL MEDIUM WITH GLUCOSE

Overview

External ontology
mediadive.medium:J1112 (source link unavailable) — MINERAL MEDIUM WITH GLUCOSE
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1112

Composition

flowchart LR
    CultureMech_002288["mineral_medium_with_glucose"]
    ing0_NaCl["NaCl"]
    CultureMech_002288 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_002288 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002288 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002288 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002288 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaSO4_x_7_H2O["CaSO4 x 7 H2O"]
    CultureMech_002288 --> ing5_CaSO4_x_7_H2O
    CHEBI_31346["CHEBI:31346"]
    ing5_CaSO4_x_7_H2O -.-> CHEBI_31346
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002288 --> ing6_Yeast_extract
    ing7_Glucose["Glucose"]
    CultureMech_002288 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Biotin["Biotin"]
    CultureMech_002288 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Folic_acid["Folic acid"]
    CultureMech_002288 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002288 --> ing10_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002288 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Riboflavin["Riboflavin"]
    CultureMech_002288 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002288 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002288 --> ing14_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_Calcium_pantothenate -.-> CHEBI_31345
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_002288 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002288 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Lipoic_acid["Lipoic acid"]
    CultureMech_002288 --> ing17_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing17_Lipoic_acid -.-> CHEBI_16494
    ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002288 --> ing18_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing19_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002288 --> ing19_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing19_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing20_Na2SeO3["Na2SeO3"]
    CultureMech_002288 --> ing20_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing20_Na2SeO3 -.-> CHEBI_48843
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002288 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002288 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002288 --> ing23_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing23_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing24_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002288 --> ing24_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing24_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002288 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002288 --> ing26_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing26_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
2.44379 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.293255 G_PER_L CHEBI:32588
potassium chloride
3 K2HPO4
HO4P.2K 174.174 g/mol
0.293255 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0977517 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.977517 G_PER_L CHEBI:31206
ammonium chloride
6 CaSO4 x 7 H2O
Ca.O4S 136.141 g/mol
0.012219 G_PER_L CHEBI:31346
calcium sulfate
7 Yeast extract 0.0977517 G_PER_L
8 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
9 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
10 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
11 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
12 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
13 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
15 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
16 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
19 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
20 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.2 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
21 Na2SeO3
2Na.O3Se 172.937 g/mol
0.1 G_PER_L CHEBI:48843
disodium selenite
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
24 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.1 G_PER_L CHEBI:86360
manganese(II) sulfate
25 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
27 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate

Preparation

  1. Mix components thoroughly, adjust pH to 7.4 and autoclave. After cooling, add the following solution (autoclaved):
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
  3. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.670063Z mediadive-import Imported from MediaDive Source: JCM, ID: J1112
2026-01-28T19:48:42.126293Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:12.015504Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002288
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.916718+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM WITH GLUCOSE" to "mineral_medium_with_glucose" for consistent matching
2026-06-05T07:41:34.407976+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.980220+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.