mrs_medium_ph_5_5

CultureMech:002293 bacterial COMPLEX LIQUID
Original name: MRS MEDIUM (pH 5.5)

Overview

External ontology
mediadive.medium:J111 (source link unavailable) — MRS MEDIUM (pH 5.5)
Applications
  • Microbial cultivation
pH
5.5
Notes
JCM Medium 111 prepares MRS Medium from JCM Medium 1 and adjusts the complete medium to pH 5.5.

Composition

flowchart LR
    CultureMech_002293["mrs_medium_ph_5_5"]
    ing0_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
    CultureMech_002293 --> ing0_Casein_peptone__tryptic_digest
    ing1_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_002293 --> ing1_Beef_extract__BD_Difco_
    ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_002293 --> ing2_Yeast_extract__BD_Difco_
    ing3_Glucose["Glucose"]
    CultureMech_002293 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Tween_80["Tween 80"]
    CultureMech_002293 --> ing4_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing4_Tween_80 -.-> CHEBI_53426
    ing5_K2HPO4["K2HPO4"]
    CultureMech_002293 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_002293 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_Diammonium_citrate["Diammonium citrate"]
    CultureMech_002293 --> ing7_Diammonium_citrate
    CHEBI_63076["CHEBI:63076"]
    ing7_Diammonium_citrate -.-> CHEBI_63076
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002293 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002293 --> ing9_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing9_MnSO4_x_n_H2O -.-> CHEBI_86360

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Casein peptone, tryptic digest 10 G_PER_L TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
2 Beef extract (BD-Difco) 10 G_PER_L TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
3 Yeast extract (BD-Difco) 5 G_PER_L TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
4 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
5 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
6 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
5 G_PER_L CHEBI:32954
sodium acetate
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
8 Diammonium citrate
C6H6O7.2H4N 226.185 g/mol
2 G_PER_L CHEBI:63076
diammonium citrate
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.
10 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.05 G_PER_L CHEBI:86360
manganese(II) sulfate
 TOGO M1 and JCM Medium 1 list this component for MRS Medium; TOGO M103/JCM Medium 111 adjust the complete medium to pH 5.5.

Preparation

  1. Dissolve the JCM Medium 1 MRS Medium components in distilled water and bring the volume to 1 L.
  2. Adjust the complete medium to pH 5.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.748334Z mediadive-import Imported from MediaDive Source: JCM, ID: J111
2026-03-13T06:19:12.059569Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002293
2026-03-17T19:45:24.966953+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS MEDIUM (pH 5.5)" to "mrs_medium_ph_5_5" for consistent matching
2026-09-07T00:00:00-07:00 repair_bacterial_togo_score40.py RESOLVED_BACTERIAL_JCM_SCORE35_GRAPH JCM Medium 111 prepares MRS Medium from JCM Medium 1 and adjusts the complete medium to pH 5.5.