ethane_c_medium

CultureMech:002294 bacterial DEFINED LIQUID
Original name: ETHANE-C MEDIUM

Overview

External ontology
mediadive.medium:J1120 (source link unavailable) — ETHANE-C MEDIUM
Applications
  • Microbial cultivation
pH
8.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1120

Composition

flowchart LR
    CultureMech_002294["ethane_c_medium"]
    ing0_NH4NO3["NH4NO3"]
    CultureMech_002294 --> ing0_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing0_NH4NO3 -.-> CHEBI_63038
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002294 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_002294 --> ing2_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing2_Fe_III__EDTA -.-> CHEBI_30729
    ing3_Vitamin_B12["Vitamin B12"]
    CultureMech_002294 --> ing3_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing3_Vitamin_B12 -.-> CHEBI_176843
    ing4_Biotin["Biotin"]
    CultureMech_002294 --> ing4_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing4_Biotin -.-> CHEBI_15956
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002294 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_002294 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002294 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002294 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_6_H2O["MnCl2 x 6 H2O"]
    CultureMech_002294 --> ing9_MnCl2_x_6_H2O
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002294 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_002294 --> ing11__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing11__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing12_NaCl["NaCl"]
    CultureMech_002294 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002294 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_KBr["KBr"]
    CultureMech_002294 --> ing14_KBr
    CHEBI_32030["CHEBI:32030"]
    ing14_KBr -.-> CHEBI_32030
    ing15_KF["KF"]
    CultureMech_002294 --> ing15_KF
    CHEBI_66872["CHEBI:66872"]
    ing15_KF -.-> CHEBI_66872
    ing16_KCl["KCl"]
    CultureMech_002294 --> ing16_KCl
    CHEBI_32588["CHEBI:32588"]
    ing16_KCl -.-> CHEBI_32588
    ing17_H3BO3["H3BO3"]
    CultureMech_002294 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002294 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19_KHCO3["KHCO3"]
    CultureMech_002294 --> ing19_KHCO3
    CHEBI_81862["CHEBI:81862"]
    ing19_KHCO3 -.-> CHEBI_81862
    ing20_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002294 --> ing20_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing20_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing21_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002294 --> ing21_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing21_MgCl2_x_6_H2O -.-> CHEBI_86345

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NH4NO3
H4N.NO3 80.043 g/mol
0.0996016 G_PER_L CHEBI:63038
ammonium nitrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.00996016 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
0.00249004 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
4 Vitamin B12 0.0011 G_PER_L CHEBI:176843
vitamin B12
5 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
6 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.2 G_PER_L CHEBI:49105
thiamine hydrochloride
7 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.372 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.00025 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00575 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 6 H2O 0.00455 G_PER_L
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.0006 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00027 G_PER_L CHEBI:91249
ammonium molybdate
13 NaCl
Cl.Na 58.443 g/mol
26.3 G_PER_L CHEBI:26710
sodium chloride
14 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.54 G_PER_L CHEBI:86158
calcium chloride dihydrate
15 KBr
Br.K 119.002 g/mol
0.1 G_PER_L CHEBI:32030
potassium bromide
16 KF
F.K 58.096 g/mol
0.004 G_PER_L CHEBI:66872
potassium fluoride
17 KCl
Cl.K 74.551 g/mol
0.7 G_PER_L CHEBI:32588
potassium chloride
18 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
19 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
20 KHCO3
CHO3.K 100.114 g/mol
0.24 G_PER_L CHEBI:81862
potassium hydrogencarbonate
21 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.017 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
22 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate

Preparation

  1. Mix components, adjust pH to 8.0 with NaOH, then distribute the medium into culture vessels (e.g., 20-50 ml per 120 ml serum bottles), seal with butyl rubber stoppers and autoclave at 110C for 5 min. Allow to stand the autoclaved medium overnight. Add ethane to the gas phase (10 to 50% of the gas phase by volume).
  2. Artificial seawater can be replaced with natural seawater. In this case, filtrate seawater (pore size, 02 �m) and boil. After cooling, filtrate again (pore size, 0.45 �m).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.701647Z mediadive-import Imported from MediaDive Source: JCM, ID: J1120
2026-01-28T19:48:36.273485Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:12.067131Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002294
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.975038+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ETHANE-C MEDIUM" to "ethane_c_medium" for consistent matching
2026-06-05T07:40:59.656499+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.725977+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.