azc_1502_medium

CultureMech:002295 bacterial COMPLEX LIQUID
Original name: AZC 1502 MEDIUM

Overview

External ontology
mediadive.medium:J1121 (source link unavailable) — AZC 1502 MEDIUM
Applications
  • Microbial cultivation
pH
5.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1121

Composition

flowchart LR
    CultureMech_002295["azc_1502_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002295 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002295 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KCl["KCl"]
    CultureMech_002295 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002295 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002295 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002295 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_002295 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002295 --> ing7_Yeast_extract
    ing8_Sodium_dithionite["Sodium dithionite"]
    CultureMech_002295 --> ing8_Sodium_dithionite
    CHEBI_66870["CHEBI:66870"]
    ing8_Sodium_dithionite -.-> CHEBI_66870
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002295 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_NiSO4_x_7_H2O["NiSO4 x 7 H2O"]
    CultureMech_002295 --> ing10_NiSO4_x_7_H2O
    CHEBI_53504["CHEBI:53504"]
    ing10_NiSO4_x_7_H2O -.-> CHEBI_53504
    ing11_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002295 --> ing11_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing11_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002295 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_HCl["HCl"]
    CultureMech_002295 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002295 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_002295 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002295 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_002295 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002295 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002295 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002295 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002295 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.0958773 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.0958773 G_PER_L CHEBI:31206
ammonium chloride
3 KCl
Cl.K 74.551 g/mol
0.0479386 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0479386 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0958773 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
3.06807 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
10 G_PER_L CHEBI:32954
sodium acetate
8 Yeast extract 10 G_PER_L
9 Sodium dithionite
2Na.O4S2 174.11 g/mol
20 G_PER_L CHEBI:66870
sodium dithionite
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.42 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 NiSO4 x 7 H2O
7H2O.Ni.O4S 280.861 g/mol
1.6 G_PER_L CHEBI:53504
nickel sulfate heptahydrate
12 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.038 G_PER_L CHEBI:63939
sodium tungstate dihydrate
13 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
14 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
15 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly and adjust pH to 5.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under the same gas atmosphere, seal with butyl rubber stoppers, and autoclave. After cooling, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture and add per liter the following solution (freshly prepared and filter-sterilized):
  2. Pressurize the inoculated culture vessels to 100 kPa H2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.705662Z mediadive-import Imported from MediaDive Source: JCM, ID: J1121
2026-03-13T06:19:12.077502Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002295
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.987615+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AZC 1502 MEDIUM" to "azc_1502_medium" for consistent matching
2026-06-05T07:40:50.124528+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.659008+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.