artficial_freshwater_medium_ii

CultureMech:002298 bacterial COMPLEX LIQUID
Original name: ARTFICIAL FRESHWATER MEDIUM II

Overview

External ontology
mediadive.medium:J1125 (source link unavailable) — ARTFICIAL FRESHWATER MEDIUM II
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1125

Composition

flowchart LR
    CultureMech_002298["artficial_freshwater_medium_ii"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002298 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002298 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KCl["KCl"]
    CultureMech_002298 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Na2SO4["Na2SO4"]
    CultureMech_002298 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_Resazurin["Resazurin"]
    CultureMech_002298 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002298 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002298 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002298 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002298 --> ing8_Yeast_extract
    ing9_glycerin["glycerin"]
    CultureMech_002298 --> ing9_glycerin
    CHEBI_17754["CHEBI:17754"]
    ing9_glycerin -.-> CHEBI_17754
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002298 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_HCl["HCl"]
    CultureMech_002298 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002298 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_ZnCl2["ZnCl2"]
    CultureMech_002298 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002298 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_002298 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002298 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002298 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002298 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002298 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NaOH["NaOH"]
    CultureMech_002298 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002298 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002298 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.19685 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.246063 G_PER_L CHEBI:31206
ammonium chloride
3 KCl
Cl.K 74.551 g/mol
0.295276 G_PER_L CHEBI:32588
potassium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
1.37795 G_PER_L CHEBI:32149
sodium sulfate
5 Resazurin
C12H7NO4 229.191 g/mol
0.000984252 G_PER_L CHEBI:8806
Resazurin
6 NaHCO3
CHO3.Na 84.006 g/mol
30 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 Yeast extract 1 G_PER_L
10 glycerin
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
  2. Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml in 60 ml-serum bottles) and seal with butyl rubber stoppers. Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2):
  3. Comment:
  4. For cultivation of JCM 31104, use 1 M sodium lactate solution instead of 1 M glycerin solution (final 10 mM).

Solutions

Vitamin solution (see Medium No. 403)

Dose: 1 G_PER_L

Thiamine solution (see Medium No. 403)

Dose: 1 G_PER_L

Vitamin B12 solution (see Medium No. 403)

Dose: 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.722356Z mediadive-import Imported from MediaDive Source: JCM, ID: J1125
2026-03-09T08:04:18.720492+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:12.113695Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002298
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.030794+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ARTFICIAL FRESHWATER MEDIUM II" to "artficial_freshwater_medium_ii" for consistent matching
2026-05-16T09:03:24.854106+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:49.679595+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.522072+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.