sulfate_free_metako_medium

CultureMech:002299 bacterial DEFINED LIQUID
Original name: SULFATE-FREE METAKO MEDIUM

Overview

External ontology
mediadive.medium:J1126 (source link unavailable) — SULFATE-FREE METAKO MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1126

Composition

flowchart LR
    CultureMech_002299["sulfate_free_metako_medium"]
    ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002299 --> ing0_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002299 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NaCl["NaCl"]
    CultureMech_002299 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_002299 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002299 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002299 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002299 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_KBr["KBr"]
    CultureMech_002299 --> ing7_KBr
    CHEBI_32030["CHEBI:32030"]
    ing7_KBr -.-> CHEBI_32030
    ing8_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002299 --> ing8_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing8_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing9_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002299 --> ing9_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing9_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing10_NaF["NaF"]
    CultureMech_002299 --> ing10_NaF
    CHEBI_28741["CHEBI:28741"]
    ing10_NaF -.-> CHEBI_28741
    ing11_LiCl["LiCl"]
    CultureMech_002299 --> ing11_LiCl
    CHEBI_48607["CHEBI:48607"]
    ing11_LiCl -.-> CHEBI_48607
    ing12_Resazurin["Resazurin"]
    CultureMech_002299 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Trimethylamine["Trimethylamine"]
    CultureMech_002299 --> ing13_Trimethylamine
    CHEBI_18139["CHEBI:18139"]
    ing13_Trimethylamine -.-> CHEBI_18139
    ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002299 --> ing14_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002299 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002299 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002299 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002299 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_ZnCl2["ZnCl2"]
    CultureMech_002299 --> ing19_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing19_ZnCl2 -.-> CHEBI_49976
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002299 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_AlCl3["AlCl3"]
    CultureMech_002299 --> ing21_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing21_AlCl3 -.-> CHEBI_30114
    ing22_H3BO3["H3BO3"]
    CultureMech_002299 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002299 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NaBr["NaBr"]
    CultureMech_002299 --> ing24_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing24_NaBr -.-> CHEBI_63004
    ing25_KI["KI"]
    CultureMech_002299 --> ing25_KI
    CHEBI_8346["CHEBI:8346"]
    ing25_KI -.-> CHEBI_8346
    ing26_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002299 --> ing26_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing26_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002299 --> ing27_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing28_NaOH["NaOH"]
    CultureMech_002299 --> ing28_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing28_NaOH -.-> CHEBI_32145
    ing29_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002299 --> ing29_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing29_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    CultureMech_002299 --> more["...11 more"]

Ingredients (41)

# Ingredient Concentration Ontology Notes
1 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
9.30255 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.33595 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 NaCl
Cl.Na 58.443 g/mol
20.3831 G_PER_L CHEBI:26710
sodium chloride
4 KCl
Cl.K 74.551 g/mol
0.589391 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
0.530452 G_PER_L CHEBI:31206
ammonium chloride
6 KH2PO4
H2O4P.K 136.084 g/mol
0.137525 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 NaHCO3
CHO3.Na 84.006 g/mol
2.64244 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 KBr
Br.K 119.002 g/mol
0.0834971 G_PER_L CHEBI:32030
potassium bromide
9 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0333988 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
10 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.0217878 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
 [Merged 2 duplicates: 0.0117878, 0.01]
11 NaF
F.Na 41.988 g/mol
0.00294695 G_PER_L CHEBI:28741
sodium fluoride
12 LiCl
Cl.Li 42.394 g/mol
0.000982318 G_PER_L CHEBI:48607
lithium chloride
13 Resazurin
C12H7NO4 229.191 g/mol
0.000982318 G_PER_L CHEBI:8806
Resazurin
14 Trimethylamine
C3H9N 59.112 g/mol
10 G_PER_L CHEBI:18139
trimethylamine
15 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
2.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
16 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
2.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.15 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.61 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.42 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 ZnCl2
2Cl.Zn 136.296 g/mol
0.09 G_PER_L CHEBI:49976
zinc dichloride
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.007 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 AlCl3
AlCl3 133.341 g/mol
0.006 G_PER_L CHEBI:30114
aluminium trichloride
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NaBr
Br.Na 102.894 g/mol
0.01 G_PER_L CHEBI:63004
sodium bromide
26 KI
I.K 166.002 g/mol
0.07 G_PER_L CHEBI:8346
potassium iodide
27 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.5 G_PER_L CHEBI:86254
iron trichloride hexahydrate
28 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
29 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
30 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
31 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
32 Biotin
C10H16N2O3S 244.316 g/mol
0.0049 G_PER_L CHEBI:15956
biotin
33 Folic acid
C19H19N7O6 441.404 g/mol
0.0088 G_PER_L CHEBI:27470
folic acid
34 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.0041 G_PER_L CHEBI:30961
pyridoxine hydrochloride
35 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0067 G_PER_L CHEBI:49105
thiamine hydrochloride
36 Riboflavin
C17H20N4O6 376.369 g/mol
0.0075 G_PER_L CHEBI:17015
riboflavin
37 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0025 G_PER_L CHEBI:15940
nicotinic acid
38 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0095 G_PER_L CHEBI:31345
Calcium pantothenate
39 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
40 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.0027 G_PER_L CHEBI:30753
4-aminobenzoic acid
41 Lipoic acid
C8H14O2S2 206.332 g/mol
0.0041 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly and distribute the medium into culture vessels (e.g., 20 ml medium in 100 ml serum bottles or 5.0 ml medium in Hungate tubes) under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Add per liter the following solution from a sterile anaerobic stock (filter-sterilized and stored under a N2 atmosphere):
  2. Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere):
  3. Comment: For strain JCM 32479, replace trimethylamine with sodium lactate (final 0.55 g/L) and yeast extract (1.0 g/L).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.726897Z mediadive-import Imported from MediaDive Source: JCM, ID: J1126
2026-03-13T03:08:49.547864Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:12.130118Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002299
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.049186+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFATE-FREE METAKO MEDIUM" to "sulfate_free_metako_medium" for consistent matching
2026-06-05T07:41:47.256373+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 39 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.934269+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.