gab_medium_for_strain_egb

CultureMech:002300 bacterial COMPLEX LIQUID
Original name: GAB MEDIUM FOR STRAIN EGB

Overview

External ontology
mediadive.medium:J1128 (source link unavailable) — GAB MEDIUM FOR STRAIN EGB
Applications
  • Microbial cultivation
pH
8.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1128

Composition

flowchart LR
    CultureMech_002300["gab_medium_for_strain_egb"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002300 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002300 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002300 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002300 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002300 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_002300 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Na2CO3["Na2CO3"]
    CultureMech_002300 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002300 --> ing7_Yeast_extract
    ing8_Ammonium_ferric_citrate["Ammonium ferric citrate"]
    CultureMech_002300 --> ing8_Ammonium_ferric_citrate
    CHEBI_31604["CHEBI:31604"]
    ing8_Ammonium_ferric_citrate -.-> CHEBI_31604

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
7 Na2CO3
CO3.2Na 105.988 g/mol
20 G_PER_L CHEBI:29377
sodium carbonate
8 Yeast extract 4 G_PER_L
9 Ammonium ferric citrate 20 G_PER_L CHEBI:31604
ferric ammonium citrate

Preparation

  1. Mix components, dispense the medium into culture vessels (e.g., 10 ml in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
  2. Adjust pH to 8.0 - 8.2, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.735178Z mediadive-import Imported from MediaDive Source: JCM, ID: J1128
2026-03-13T06:19:12.139607Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002300
2026-03-17T06:30:28.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.060708+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GAB MEDIUM FOR STRAIN EGB" to "gab_medium_for_strain_egb" for consistent matching
2026-06-05T07:41:05.868185+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.933116+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.