Overview
- External ontology
- mediadive.medium:J112 (source link unavailable) — CM3 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=112
Composition
flowchart LR
CultureMech_002302["cm3_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002302 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_Urea["Urea"]
CultureMech_002302 --> ing1_Urea
CHEBI_16199["CHEBI:16199"]
ing1_Urea -.-> CHEBI_16199
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002302 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002302 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002302 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002302 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002302 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Yeast_extract["Yeast extract"]
CultureMech_002302 --> ing7_Yeast_extract
ing8_MOPS["MOPS"]
CultureMech_002302 --> ing8_MOPS
CHEBI_39074["CHEBI:39074"]
ing8_MOPS -.-> CHEBI_39074
ing9_Cellobiose_or_Cellulose_MN_300["Cellobiose or Cellulose MN 300"]
CultureMech_002302 --> ing9_Cellobiose_or_Cellulose_MN_300
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
Urea
CH4N2O 60.056 g/mol
|
1.5 G_PER_L |
CHEBI:16199
urea
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 | Yeast extract | 6 G_PER_L | ||
| 9 | MOPS | 20 G_PER_L |
CHEBI:39074
MOPS
|
|
| 10 | Cellobiose or Cellulose MN 300 | 10 G_PER_L |
Preparation
- Adjust pH to 7.1.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.749493Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J112 |
| 2026-03-13T06:19:12.154959Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002302 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.079168+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM3 MEDIUM" to "cm3_medium" for consistent matching |
| 2026-06-05T07:40:54.510805+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.919025+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |