Overview
- External ontology
- mediadive.medium:J1132 (source link unavailable) — HALOBACTERIA MEDIUM (pH 8.0)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1132 These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. The captured MediaDive J1132 provider dataset identifies this preparation; the original JCM1132 page returned Nothing found. This is not a recovered JCM original or a DOI-backed publication.
Ingredients (0)
No ingredients recorded.
Preparation
- Adjust pH to 8.0.
Solutions
Main sol. J168
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Casamino acids | 5 G_PER_L | 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| Yeast extract | 5 G_PER_L | 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| Na glutamate | 1 G_PER_L | 1.0 g in the source preparation brought to 1.0 L gives 1 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| Trisodium citrate | 3 G_PER_L | 3.0 g in the source preparation brought to 1.0 L gives 3 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| MgSO4 x 7 H2O | 20 G_PER_L | 20.0 g in the source preparation brought to 1.0 L gives 20 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| KCl | 2 G_PER_L | 2.0 g in the source preparation brought to 1.0 L gives 2 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| NaCl | 200 G_PER_L | 200.0 g in the source preparation brought to 1.0 L gives 200 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| FeCl2 x 4 H2O | 36 MG_PER_L | 36.0 mg in the source preparation brought to 1.0 L gives 36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| MnCl2 x 4 H2O | 0.36 MG_PER_L | 0.36 mg in the source preparation brought to 1.0 L gives 0.36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| distilled water | Water makes the preparation up to 1.0 L. Its separate input volume and mass are unspecified; neither 1 g/L nor a fixed 1 L water dose is supported. These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.750262Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1132 |
| 2026-01-28T19:48:10.856766Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:12.185541Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002305 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.115423+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTERIA MEDIUM (pH 8.0)" to "halobacteria_medium_ph_8_0" for consistent matching |
| 2026-06-05T07:41:07.544249+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.644230+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-09T14:23:51.976655+00:00 | codex | CURATED_HALOBACTERIA_VARIANT_CONCENTRATIONS_AND_PREPARATION_STAGES | Source-specific ingredient and variant review. Every change has an exact PURL-backed excerpt. Prepared-base quantities, unquantified water/titrant, source limitations, chemical identities and prior history are preserved; no taxa-media assertions are added. |