pyg_medium_i

CultureMech:002306 bacterial COMPLEX LIQUID
Original name: PYG MEDIUM (I)

Overview

External ontology
mediadive.medium:J1133 (source link unavailable) — PYG MEDIUM (I)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1133

Composition

flowchart LR
    CultureMech_002306["pyg_medium_i"]
    ing0_Peptone["Peptone"]
    CultureMech_002306 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002306 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_002306 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002306 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002306 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002306 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002306 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Peptone 4 G_PER_L
2 Yeast extract 1 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
4 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate

Preparation

  1. Mix components and adjust pH to 7.0. Dispense the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.752600Z mediadive-import Imported from MediaDive Source: JCM, ID: J1133
2026-03-13T06:19:12.190176Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002306
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.121080+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYG MEDIUM (I)" to "pyg_medium_i" for consistent matching
2026-06-05T07:41:43.082162+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.595270+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.