Overview
- External ontology
- mediadive.medium:J1137 (source link unavailable) — ROSEIBACA CALIDILACUS MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1137
Composition
flowchart LR
CultureMech_002310["roseibaca_calidilacus_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_002310 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Glycerol["Glycerol"]
CultureMech_002310 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_KH2PO4["KH2PO4"]
CultureMech_002310 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Na2CO3["Na2CO3"]
CultureMech_002310 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Ammonium_ferric_citrate["Ammonium ferric citrate"]
CultureMech_002310 --> ing4_Ammonium_ferric_citrate
CHEBI_31604["CHEBI:31604"]
ing4_Ammonium_ferric_citrate -.-> CHEBI_31604
ing5_TES["TES"]
CultureMech_002310 --> ing5_TES
CHEBI_39035["CHEBI:39035"]
ing5_TES -.-> CHEBI_39035
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002310 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Na2SO4["Na2SO4"]
CultureMech_002310 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_KCl["KCl"]
CultureMech_002310 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaNO3["NaNO3"]
CultureMech_002310 --> ing9_NaNO3
CHEBI_63005["CHEBI:63005"]
ing9_NaNO3 -.-> CHEBI_63005
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002310 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_Na2_EDTA["Na2-EDTA"]
CultureMech_002310 --> ing11_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing11_Na2_EDTA -.-> CHEBI_64734
ing12_H3BO3["H3BO3"]
CultureMech_002310 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002310 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002310 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002310 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002310 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_Ca_NO3_2_x_6_H2O["Ca(NO3)2 x 6 H2O"]
CultureMech_002310 --> ing17_Ca_NO3_2_x_6_H2O
ing18_Biotin["Biotin"]
CultureMech_002310 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Folic_acid["Folic acid"]
CultureMech_002310 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002310 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Thiamine_HCl["Thiamine HCl"]
CultureMech_002310 --> ing21_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamine_HCl -.-> CHEBI_49105
ing22_Riboflavin["Riboflavin"]
CultureMech_002310 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002310 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002310 --> ing24_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_pantothenate -.-> CHEBI_31345
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_002310 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002310 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Lipoic_acid["Lipoic acid"]
CultureMech_002310 --> ing27_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27_Lipoic_acid -.-> CHEBI_16494
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.84 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
0.46 G_PER_L |
CHEBI:17754
glycerol
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.03 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.02 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 | Ammonium ferric citrate | 0.00142 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 6 | TES | 10 G_PER_L |
CHEBI:39035
TES
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
500.49 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 493.0, 7.49] |
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
57 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
7.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NaNO3
NO3.Na 84.994 g/mol
|
149.58 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.1 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.079 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 | Ca(NO3)2 x 6 H2O | 0.0494 G_PER_L | ||
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 22 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly and filter-sterilize.
- Add components to distilled water and bring volume to 1.0 L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.765042Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1137 |
| 2026-03-13T03:09:18.778232Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:12.226524Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002310 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.164641+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ROSEIBACA CALIDILACUS MEDIUM" to "roseibaca_calidilacus_medium" for consistent matching |
| 2026-06-05T07:41:44.582248+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.049913+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |