Overview
- External ontology
- mediadive.medium:J1138 (source link unavailable) — NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1138
Composition
flowchart LR
CultureMech_002311["neutral_oligotrophic_haloarchaeal_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002311 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_002311 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Yeast_extract["Yeast extract"]
CultureMech_002311 --> ing2_Yeast_extract
ing3_Peptone["Peptone"]
CultureMech_002311 --> ing3_Peptone
ing4_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002311 --> ing4_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Sodium_pyruvate -.-> CHEBI_50144
ing5_NaCl["NaCl"]
CultureMech_002311 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002311 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002311 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_KCl["KCl"]
CultureMech_002311 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_CaCl2["CaCl2"]
CultureMech_002311 --> ing9_CaCl2
CHEBI_3312["CHEBI:3312"]
ing9_CaCl2 -.-> CHEBI_3312
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 | Yeast extract | 0.05 G_PER_L | ||
| 4 | Peptone | 0.25 G_PER_L | ||
| 5 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
30 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
35 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5549 G_PER_L |
CHEBI:3312
calcium dichloride
|
Preparation
- Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574).
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.769767Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1138 |
| 2026-03-13T06:19:12.234398Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002311 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.173947+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM" to "neutral_oligotrophic_haloarchaeal_medium" for consistent matching |
| 2026-06-05T07:41:39.600790+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.526193+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |