Overview
- External ontology
- mediadive.medium:J1140 (source link unavailable) — LONGIMONAS HALOALKALIPHILA MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1140
Composition
flowchart LR
CultureMech_002314["longimonas_haloalkaliphila_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_002314 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002314 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002314 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_002314 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_002314 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_MgCl2["MgCl2"]
CultureMech_002314 --> ing5_MgCl2
CHEBI_6636["CHEBI:6636"]
ing5_MgCl2 -.-> CHEBI_6636
ing6_Na2CO3["Na2CO3"]
CultureMech_002314 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_NaHCO3["NaHCO3"]
CultureMech_002314 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_NH4Cl["NH4Cl"]
CultureMech_002314 --> ing8_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing8_NH4Cl -.-> CHEBI_31206
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 15 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
122285.71 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 120000.0, 2285.71] |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1535.714 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
[Merged 2 duplicates: 1250.0, 285.714] |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
250 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 6 |
MgCl2
2Cl.Mg 95.211 g/mol
|
1.0 G_PER_L |
CHEBI:6636
magnesium dichloride
|
[Collapsed 2 identical duplicates: 1.0, 1.0] |
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
27142.9 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4285.71 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
NH4Cl
Cl.H4N 53.492 g/mol
|
4 G_PER_L |
CHEBI:31206
ammonium chloride
|
Preparation
- Aseptically combine the above described media and solutions.
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 - 6.9 by 1 M K2HPO4 and autoclave. After cooling, add the following solutions (autoclaved):
- Add components to distilled water and bring volume to 1.0 L. Check pH of the medium to be 9.9 - 10.0. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.776687Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1140 |
| 2026-03-13T03:09:35.917671Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:12.252494Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002314 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.194508+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LONGIMONAS HALOALKALIPHILA MEDIUM" to "longimonas_haloalkaliphila_medium" for consistent matching |
| 2026-06-05T07:41:11.383009+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.575151+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:12.726875+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |