Overview
- External ontology
- mediadive.medium:J1146 (source link unavailable) — METHANIMICROCOCCUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1146
Composition
flowchart LR
CultureMech_002319["methanimicrococcus_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002319 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_002319 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_002319 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002319 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002319 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NaCl["NaCl"]
CultureMech_002319 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002319 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Yeast_extract["Yeast extract"]
CultureMech_002319 --> ing7_Yeast_extract
ing8_Casitone["Casitone"]
CultureMech_002319 --> ing8_Casitone
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_002319 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Resazurin["Resazurin"]
CultureMech_002319 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_NaHCO3["NaHCO3"]
CultureMech_002319 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Methanol["Methanol"]
CultureMech_002319 --> ing12_Methanol
CHEBI_17790["CHEBI:17790"]
ing12_Methanol -.-> CHEBI_17790
ing13_Coenzyme_M["Coenzyme M"]
CultureMech_002319 --> ing13_Coenzyme_M
CHEBI_17905["CHEBI:17905"]
ing13_Coenzyme_M -.-> CHEBI_17905
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002319 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002319 --> ing15_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing15_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing16_HCl["HCl"]
CultureMech_002319 --> ing16_HCl
CHEBI_17883["CHEBI:17883"]
ing16_HCl -.-> CHEBI_17883
ing17_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002319 --> ing17_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing17_FeCl2_x_4_H2O -.-> CHEBI_86249
ing18_ZnCl2["ZnCl2"]
CultureMech_002319 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002319 --> ing19_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
ing20_H3BO3["H3BO3"]
CultureMech_002319 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002319 --> ing21_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002319 --> ing22_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002319 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002319 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing25_Biotin["Biotin"]
CultureMech_002319 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_002319 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002319 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_002319 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_002319 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_002319 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.344488 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.226378 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.492126 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.492126 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.246063 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
2.21457 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0019685 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 | Yeast extract | 1.9685 G_PER_L | ||
| 9 | Casitone | 1.9685 G_PER_L | ||
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.984252 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000492126 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
35 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Methanol
CH4O 32.042 g/mol
|
20 G_PER_L |
CHEBI:17790
methanol
|
|
| 14 |
Coenzyme M
C2H6O3S2 142.201 g/mol
|
10 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
7.2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
7.2 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 17 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 18 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 20 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 23 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved or filter-sterilized):
- Aseptically and anaerobically distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas stream and seal with butyl stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
- Readjust pH to 7.0 - 7.2, if necessary. Pressurize inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.798562Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1146 |
| 2026-03-13T06:19:12.314225Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002319 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.264922+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANIMICROCOCCUS MEDIUM" to "methanimicrococcus_medium" for consistent matching |
| 2026-06-05T07:41:30.555266+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.603936+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |