nitrososphaera_gargensis_medium

Overview

External ontology

mediadive.medium:J1148 (source link unavailable) — NITROSOSPHAERA GARGENSIS MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1148

Composition

flowchart LR
    CultureMech_002321["nitrososphaera_gargensis_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002321 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_KCl["KCl"]
    CultureMech_002321 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002321 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_002321 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCO3["CaCO3"]
    CultureMech_002321 --> ing4_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing4_CaCO3 -.-> CHEBI_3311
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002321 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_HCl["HCl"]
    CultureMech_002321 --> ing6_HCl
    CHEBI_17883["CHEBI:17883"]
    ing6_HCl -.-> CHEBI_17883
    ing7_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
    CultureMech_002321 --> ing7_MnSO4_x_5_H2O
    CHEBI_131524["CHEBI:131524"]
    ing7_MnSO4_x_5_H2O -.-> CHEBI_131524
    ing8_H3BO3["H3BO3"]
    CultureMech_002321 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_ZnCl2["ZnCl2"]
    CultureMech_002321 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002321 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002321 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002321 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002321 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002321 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_NaOH["NaOH"]
    CultureMech_002321 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145
    ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002321 --> ing16_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002321 --> ing17_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (18)

Preparation

1. Mix components and autoclave (CaCO3 will not fully dissolve). After cooling, add the following solutions to the medium (autoclaved or *filter-sterilized):
2. Comments: Typically, prepare the medium in glass bottles with plastic caps leaving an ample head space (e.g., 50 ml medium in 250 ml-Duran&reg; glass bottles). Cultivate in the dark without agitation. Check growth by phase-contrast microscopy. Also, it can be checked by measuring ammonium concentration. Once ammonium in the medium is depleted, it can be fed by the addition of another 50 mM NH4Cl solution (final 1.0 mM). The ammonium-feeding can be repeated several times. If the culture stops the ammonium oxidation or begins to grow even slower, nitrite may be accumulated up to a level causing growth inhibition in the culture (ca. 15 mM). If so, replace the medium as follows: spin down the culture (< 65,000 g for 15 min), remove the supernatant, and add fresh culture medium to the white pellet. Then, suspend the pellet and remove the suspension to a new glass bottle.

Target organisms

No target organisms are recorded.

Provenance