Overview
- External ontology
- mediadive.medium:J114 (source link unavailable) — MICROTETRASPORA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=114
Composition
flowchart LR
CultureMech_002323["microtetraspora_medium"]
ing0_Glucose["Glucose"]
CultureMech_002323 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_L_Asparagine["L-Asparagine"]
CultureMech_002323 --> ing1_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing1_L_Asparagine -.-> CHEBI_17196
ing2_K2HPO4["K2HPO4"]
CultureMech_002323 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002323 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002323 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002323 --> ing5_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing5_MnSO4_x_n_H2O -.-> CHEBI_86360
ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002323 --> ing6_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002323 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_Agar["Agar"]
CultureMech_002323 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17196
L-asparagine
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.001 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 7 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.001 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.001 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.752743Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J114 |
| 2026-03-13T06:19:12.355785Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002323 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.315754+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MICROTETRASPORA MEDIUM" to "microtetraspora_medium" for consistent matching |
| 2026-06-05T07:41:33.845681+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.818860+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |