k_medium

CultureMech:002326 bacterial DEFINED SOLID_AGAR
Original name: K MEDIUM

Overview

External ontology
mediadive.medium:J1154 (source link unavailable) — K MEDIUM
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1154

Composition

flowchart LR
    CultureMech_002326["k_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002326 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002326 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NaCl["NaCl"]
    CultureMech_002326 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002326 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002326 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_Methanol["Methanol"]
    CultureMech_002326 --> ing5_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing5_Methanol -.-> CHEBI_17790
    ing6_Agar["Agar"]
    CultureMech_002326 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
0.5 G_PER_L CHEBI:26710
sodium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.125 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.02 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 Methanol
CH4O 32.042 g/mol
10 G_PER_L CHEBI:17790
methanol
7 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.4. After autoclaving, aseptically add filter-sterilized methanol.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.828871Z mediadive-import Imported from MediaDive Source: JCM, ID: J1154
2026-03-13T06:19:12.377494Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002326
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.341208+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "K MEDIUM" to "k_medium" for consistent matching
2026-06-05T07:40:16.568354+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.747075+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.