desulfonatronum_par180_medium

CultureMech:002329 bacterial COMPLEX LIQUID
Original name: DESULFONATRONUM PAR180 MEDIUM

Overview

External ontology
mediadive.medium:J1157 (source link unavailable) — DESULFONATRONUM PAR180 MEDIUM
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1157

Composition

flowchart LR
    CultureMech_002329["desulfonatronum_par180_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002329 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002329 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002329 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_Na2CO3["Na2CO3"]
    CultureMech_002329 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002329 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002329 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_002329 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002329 --> ing7_Yeast_extract
    ing8_Sodium_lactate["Sodium lactate"]
    CultureMech_002329 --> ing8_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing8_Sodium_lactate -.-> CHEBI_75228
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002329 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002329 --> ing10_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing10_Thiamine_HCl -.-> CHEBI_49105
    ing11_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002329 --> ing11_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing11_Nicotinic_acid -.-> CHEBI_15940
    ing12_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002329 --> ing12_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing12_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002329 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Biotin["Biotin"]
    CultureMech_002329 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002329 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_002329 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_HCl["HCl"]
    CultureMech_002329 --> ing17_HCl
    CHEBI_17883["CHEBI:17883"]
    ing17_HCl -.-> CHEBI_17883
    ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002329 --> ing18_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing19_ZnCl2["ZnCl2"]
    CultureMech_002329 --> ing19_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing19_ZnCl2 -.-> CHEBI_49976
    ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002329 --> ing20_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing21_H3BO3["H3BO3"]
    CultureMech_002329 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002329 --> ing22_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing23_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002329 --> ing23_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing23_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002329 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002329 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002329 --> ing26_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing26_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing27_NiSO4_x_7_H2O["NiSO4 x 7 H2O"]
    CultureMech_002329 --> ing27_NiSO4_x_7_H2O
    CHEBI_53504["CHEBI:53504"]
    ing27_NiSO4_x_7_H2O -.-> CHEBI_53504
    ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002329 --> ing28_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing29_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002329 --> ing29_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing29_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
2.46063 G_PER_L CHEBI:26710
sodium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.0984252 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Na2SO4
2Na.O4S 142.043 g/mol
1.9685 G_PER_L CHEBI:32149
sodium sulfate
4 Na2CO3
CO3.2Na 105.988 g/mol
1.72244 G_PER_L CHEBI:29377
sodium carbonate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.123031 G_PER_L CHEBI:31206
ammonium chloride
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0492126 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 KCl
Cl.K 74.551 g/mol
0.0984252 G_PER_L CHEBI:32588
potassium chloride
8 Yeast extract 0.0984252 G_PER_L
9 Sodium lactate
C3H5O3.Na 112.06 g/mol
20 G_PER_L CHEBI:75228
sodium lactate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
8 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.00492126 G_PER_L CHEBI:49105
thiamine hydrochloride
12 Nicotinic acid
C6H5NO2 123.111 g/mol
0.00984252 G_PER_L CHEBI:15940
nicotinic acid
13 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.00246063 G_PER_L CHEBI:31345
Calcium pantothenate
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.0246063 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Biotin
C10H16N2O3S 244.316 g/mol
0.000984252 G_PER_L CHEBI:15956
biotin
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.00492126 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Vitamin B12 0.00984252 G_PER_L CHEBI:176843
vitamin B12
18 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
19 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
20 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
21 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
22 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
23 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
24 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
27 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.42 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
28 NiSO4 x 7 H2O
7H2O.Ni.O4S 280.861 g/mol
1.6 G_PER_L CHEBI:53504
nickel sulfate heptahydrate
29 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.038 G_PER_L CHEBI:63939
sodium tungstate dihydrate
30 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Mix components thoroughly and adjust pH to 9.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
  2. Vitamin solution:

Solutions

Vitamin solution

Dose: 1 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.836221Z mediadive-import Imported from MediaDive Source: JCM, ID: J1157
2026-03-09T08:04:41.217389+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:12.402126Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002329
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.369663+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFONATRONUM PAR180 MEDIUM" to "desulfonatronum_par180_medium" for consistent matching
2026-05-16T09:03:29.656196+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:56.939763+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.705012+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.