ghiorsea_bivora_medium

CultureMech:002330 bacterial DEFINED LIQUID
Original name: GHIORSEA BIVORA MEDIUM

Overview

External ontology
mediadive.medium:J1158 (source link unavailable) — GHIORSEA BIVORA MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1158

Composition

flowchart LR
    CultureMech_002330["ghiorsea_bivora_medium"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_002330 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_Modified_trace_vitamins["Modified trace vitamins"]
    CultureMech_002330 --> ing1_Modified_trace_vitamins
    ing2_Biotin["Biotin"]
    CultureMech_002330 --> ing2_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing2_Biotin -.-> CHEBI_15956
    ing3_Folic_acid["Folic acid"]
    CultureMech_002330 --> ing3_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing3_Folic_acid -.-> CHEBI_27470
    ing4_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002330 --> ing4_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing4_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002330 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Riboflavin["Riboflavin"]
    CultureMech_002330 --> ing6_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing6_Riboflavin -.-> CHEBI_17015
    ing7_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002330 --> ing7_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing7_Nicotinic_acid -.-> CHEBI_15940
    ing8_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002330 --> ing8_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing8_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_002330 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002330 --> ing10_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing10_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing11_Lipoic_acid["Lipoic acid"]
    CultureMech_002330 --> ing11_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing11_Lipoic_acid -.-> CHEBI_16494
    ing12_KH2PO4["KH2PO4"]
    CultureMech_002330 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_NaCl["NaCl"]
    CultureMech_002330 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002330 --> ing14_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing14_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002330 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_KCl["KCl"]
    CultureMech_002330 --> ing16_KCl
    CHEBI_32588["CHEBI:32588"]
    ing16_KCl -.-> CHEBI_32588
    ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002330 --> ing17_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing18_NH4Cl["NH4Cl"]
    CultureMech_002330 --> ing18_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing18_NH4Cl -.-> CHEBI_31206
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002330 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2SeO3["Na2SeO3"]
    CultureMech_002330 --> ing20_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing20_Na2SeO3 -.-> CHEBI_48843
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002330 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002330 --> ing22_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing23_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002330 --> ing23_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing23_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002330 --> ing24_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002330 --> ing25_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002330 --> ing26_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002330 --> ing27_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing28_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002330 --> ing28_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing28_AlK_SO4_2 -.-> CHEBI_86463
    ing29_H3BO3["H3BO3"]
    CultureMech_002330 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_002330 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
1.039161 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 [Merged 2 duplicates: 0.839161, 0.2]
2 Modified trace vitamins 1 G_PER_L
3 Biotin
C10H16N2O3S 244.316 g/mol
0.000999001 G_PER_L CHEBI:15956
biotin
4 Folic acid
C19H19N7O6 441.404 g/mol
0.000999001 G_PER_L CHEBI:27470
folic acid
5 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.00499501 G_PER_L CHEBI:30961
pyridoxine hydrochloride
6 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0024975 G_PER_L CHEBI:49105
thiamine hydrochloride
7 Riboflavin
C17H20N4O6 376.369 g/mol
0.0024975 G_PER_L CHEBI:17015
riboflavin
8 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0024975 G_PER_L CHEBI:15940
nicotinic acid
9 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0024975 G_PER_L CHEBI:31345
Calcium pantothenate
10 Vitamin B12 4.995e-05 G_PER_L CHEBI:176843
vitamin B12
11 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.0024975 G_PER_L CHEBI:30753
4-aminobenzoic acid
12 Lipoic acid
C8H14O2S2 206.332 g/mol
0.0024975 G_PER_L CHEBI:16494
lipoic acid
13 KH2PO4
H2O4P.K 136.084 g/mol
0.49955 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 [Merged 2 duplicates: 0.44955, 0.05]
14 NaCl
Cl.Na 58.443 g/mol
28.5 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 27.5, 1.0]
15 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.38 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
9.780000000000001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 6.78, 3.0]
17 KCl
Cl.K 74.551 g/mol
0.72 G_PER_L CHEBI:32588
potassium chloride
18 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 1.4, 0.1]
19 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
21 Na2SeO3
2Na.O3Se 172.937 g/mol
0.001 G_PER_L CHEBI:48843
disodium selenite
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.01 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
24 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
25 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
26 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
27 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
28 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
29 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
30 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
31 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly. Gas with N2-CO2 (4:1, v/v) to establish pH 6.5. Dispense the medium in culture vessels (e.g., 20 ml in 30 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling add per liter the following solution (filter-sterilized):
  2. Prior to inoculation, add filter-sterilized 5% H2 and 5% air to the gas phase (by volume). Incubate statically. Replenish H2 and air every two days.
  3. Modified trace vitamins:
  4. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.840826Z mediadive-import Imported from MediaDive Source: JCM, ID: J1158
2026-03-13T03:09:17.489357Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:12.416602Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002330
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.385921+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GHIORSEA BIVORA MEDIUM" to "ghiorsea_bivora_medium" for consistent matching
2026-06-05T07:41:06.332122+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.091402+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.