mollicutes_h_rf3_medium

CultureMech:002331 bacterial COMPLEX LIQUID
Original name: MOLLICUTES H-RF3 MEDIUM

Overview

External ontology
mediadive.medium:J1159 (source link unavailable) — MOLLICUTES H-RF3 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1159

Composition

flowchart LR
    CultureMech_002331["mollicutes_h_rf3_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002331 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002331 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002331 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002331 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Casamino_acids["Casamino acids"]
    CultureMech_002331 --> ing4_Casamino_acids
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002331 --> ing5_Yeast_extract
    ing6_Resazurin["Resazurin"]
    CultureMech_002331 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002331 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002331 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002331 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002331 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002331 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002331 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_NaCl["NaCl"]
    CultureMech_002331 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002331 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002331 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002331 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002331 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002331 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_002331 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002331 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Biotin["Biotin"]
    CultureMech_002331 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_002331 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002331 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002331 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_002331 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002331 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002331 --> ing27_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_002331 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002331 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_002331 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.132953 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.189934 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.24245 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.14245, 0.1]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.512821 G_PER_L CHEBI:31206
ammonium chloride
5 Casamino acids 0.949668 G_PER_L
6 Yeast extract 0.949668 G_PER_L
7 Resazurin
C12H7NO4 229.191 g/mol
0.000474834 G_PER_L CHEBI:8806
Resazurin
8 NaHCO3
CHO3.Na 84.006 g/mol
30 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
13 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
14 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
28 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
29 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components and adjust pH to 7.5. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
  2. Readjust pH to 7.0, if necessary.
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.846603Z mediadive-import Imported from MediaDive Source: JCM, ID: J1159
2026-01-28T19:48:38.313245Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:36.058985Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:12.431020Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002331
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.402469+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MOLLICUTES H-RF3 MEDIUM" to "mollicutes_h_rf3_medium" for consistent matching
2026-06-05T07:41:38.123972+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.207994+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:09.066475+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.