cyclonatrum_proteilyticum_medium

CultureMech:002334 bacterial COMPLEX LIQUID
Original name: CYCLONATRUM PROTEILYTICUM MEDIUM

Overview

External ontology
mediadive.medium:J1161 (source link unavailable) — CYCLONATRUM PROTEILYTICUM MEDIUM
Applications
  • Microbial cultivation
pH
9.5
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1161

Composition

flowchart LR
    CultureMech_002334["cyclonatrum_proteilyticum_medium"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_002334 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_002334 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002334 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgCl2["MgCl2"]
    CultureMech_002334 --> ing3_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing3_MgCl2 -.-> CHEBI_6636
    ing4_Tryptone["Tryptone"]
    CultureMech_002334 --> ing4_Tryptone
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002334 --> ing5_Yeast_extract
    ing6_EDTA["EDTA"]
    CultureMech_002334 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002334 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002334 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002334 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_002334 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002334 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002334 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002334 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002334 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Biotin["Biotin"]
    CultureMech_002334 --> ing15_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotin -.-> CHEBI_15956
    ing16_Folic_acid["Folic acid"]
    CultureMech_002334 --> ing16_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing16_Folic_acid -.-> CHEBI_27470
    ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002334 --> ing17_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing18_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002334 --> ing18_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing18_Thiamine_HCl -.-> CHEBI_49105
    ing19_Riboflavin["Riboflavin"]
    CultureMech_002334 --> ing19_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing19_Riboflavin -.-> CHEBI_17015
    ing20_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002334 --> ing20_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_acid -.-> CHEBI_15940
    ing21_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002334 --> ing21_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing21_Calcium_pantothenate -.-> CHEBI_31345
    ing22_Vitamin_B12["Vitamin B12"]
    CultureMech_002334 --> ing22_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing22_Vitamin_B12 -.-> CHEBI_176843
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002334 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24_Lipoic_acid["Lipoic acid"]
    CultureMech_002334 --> ing24_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing24_Lipoic_acid -.-> CHEBI_16494

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
1750 G_PER_L CHEBI:29377
sodium carbonate
2 NaHCO3
CHO3.Na 84.006 g/mol
1250 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 K2HPO4
HO4P.2K 174.174 g/mol
50 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgCl2
2Cl.Mg 95.211 g/mol
1 G_PER_L CHEBI:6636
magnesium dichloride
5 Tryptone 15 G_PER_L
6 Yeast extract 2 G_PER_L
7 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
17 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
18 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
19 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
20 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
21 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
22 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
23 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
24 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
25 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 9.5 with HCl and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
  2. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.856116Z mediadive-import Imported from MediaDive Source: JCM, ID: J1161
2026-03-13T06:19:12.462381Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002334
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.438976+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CYCLONATRUM PROTEILYTICUM MEDIUM" to "cyclonatrum_proteilyticum_medium" for consistent matching
2026-06-05T07:40:55.176614+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.089462+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.