modified_baars_medium

CultureMech:002336 bacterial COMPLEX LIQUID
Original name: MODIFIED BAAR'S MEDIUM

Overview

External ontology
mediadive.medium:J1163 (source link unavailable) — MODIFIED BAAR'S MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1163

Composition

flowchart LR
    CultureMech_002336["modified_baars_medium"]
    ing0_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_002336 --> ing0_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing0_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002336 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaSO4["CaSO4"]
    CultureMech_002336 --> ing2_CaSO4
    CHEBI_31346["CHEBI:31346"]
    ing2_CaSO4 -.-> CHEBI_31346
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002336 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002336 --> ing4_Yeast_extract
    ing5__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_002336 --> ing5__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing5__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing6_Sodium_lactate["Sodium lactate"]
    CultureMech_002336 --> ing6_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing6_Sodium_lactate -.-> CHEBI_75228

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
5 G_PER_L CHEBI:53258
sodium citrate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaSO4
Ca.O4S 136.141 g/mol
1 G_PER_L CHEBI:31346
calcium sulfate
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
5 Yeast extract 1 G_PER_L
6 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
1 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
7 Sodium lactate
C3H5O3.Na 112.06 g/mol
3.5 G_PER_L CHEBI:75228
sodium lactate

Preparation

  1. Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down and dispense the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under a N2 gas stream, seal with butyl rubber stoppers and autoclave.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.864021Z mediadive-import Imported from MediaDive Source: JCM, ID: J1163
2026-01-28T19:48:08.551933Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:12.479975Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002336
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.460566+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED BAAR'S MEDIUM" to "modified_baars_medium" for consistent matching
2026-06-05T07:41:36.224674+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.531280+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.