Overview
- External ontology
- mediadive.medium:J1168 (source link unavailable) — LB (LURIA-BERTANI) AGAR WITH KANAMYCIN AND RIFAMPICIN
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1168
Composition
flowchart LR
CultureMech_002340["lb_luria_bertani_agar_with_kanamycin_and_rifampicin"]
ing0_Tryptone["Tryptone"]
CultureMech_002340 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002340 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002340 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar["Agar"]
CultureMech_002340 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Kanamycin_sulfate["Kanamycin sulfate"]
CultureMech_002340 --> ing4_Kanamycin_sulfate
CHEBI_6109["CHEBI:6109"]
ing4_Kanamycin_sulfate -.-> CHEBI_6109
ing5_Rifampicin["Rifampicin"]
CultureMech_002340 --> ing5_Rifampicin
CHEBI_28077["CHEBI:28077"]
ing5_Rifampicin -.-> CHEBI_28077
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
Kanamycin sulfate
C18H36N4O11.H2O4S 582.582 g/mol
|
0.05 G_PER_L |
CHEBI:6109
kanamycin A sulfate
|
|
| 6 |
Rifampicin
C43H58N4O12 822.953 g/mol
|
0.025 G_PER_L |
CHEBI:28077
rifampicin
|
Preparation
- Adjust pH to 7.0.
- After autoclaving, aseptically add the following antibiotics (filter-sterilized):
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.878945Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1168 |
| 2026-03-13T06:19:12.515447Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002340 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.503233+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB (LURIA-BERTANI) AGAR WITH KANAMYCIN AND RIFAMPICIN" to "lb_luria_bertani_agar_with_kanamycin_and_rifampicin" for consistent matching |
| 2026-06-05T07:41:10.894490+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |