Overview
- External ontology
- mediadive.medium:J1170 (source link unavailable) — A7 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1170
Composition
flowchart LR
CultureMech_002343["a7_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002343 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_002343 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Urea["Urea"]
CultureMech_002343 --> ing2_Urea
CHEBI_16199["CHEBI:16199"]
ing2_Urea -.-> CHEBI_16199
ing3_Yeast_extract["Yeast extract"]
CultureMech_002343 --> ing3_Yeast_extract
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002343 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na2CO3["Na2CO3"]
CultureMech_002343 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002343 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Resazurin["Resazurin"]
CultureMech_002343 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Cellobiose["Cellobiose"]
CultureMech_002343 --> ing8_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing8_Cellobiose -.-> CHEBI_17057
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002343 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002343 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Urea
CH4N2O 60.056 g/mol
|
2.1 G_PER_L |
CHEBI:16199
urea
|
|
| 4 | Yeast extract | 6 G_PER_L | ||
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
37.51 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.01, 37.5] |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Cellobiose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
25 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Preparation
- Adjust pH to 9.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.883352Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1170 |
| 2026-03-13T03:09:30.021998Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:12.528072Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002343 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.518315+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A7 MEDIUM" to "a7_medium" for consistent matching |
| 2026-06-05T07:40:46.455460+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.301480+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |